Elsevier

Theriogenology

Volume 44, Issue 8, December 1995, Pages 1167-1189
Theriogenology

Morphological and physiological differences between in vivo- and in vitro-produced preimplantation embryos from livestock species

https://doi.org/10.1016/0093-691X(95)00327-5Get rights and content

Abstract

Almost fifty years have passed since 1949 when Dowling (14) used an egg-saline medium, developed by Hammond (26) for mouse embryos to culture 8-cell bovine embryos to the 16-cell stage. These investigators certainly must have queried the normality of the cultured embryos with their variations in cell shape and color observed. With the advances of in vitro systems and the improvement of instrumentation, investigators are faced with the same fundamental questions asked some fifty years ago. Namely, how viable are embryos that are manipulated in vitro, as compared to embryos in vivo? Compounding this difficult question is the lack of basic information on metabolic processes of in vivo embryos. Once the embryos are removed from the maternal environment for study, they are exposed to myriad in vitro conditions which can negatively effect metabolism or morphology. This manuscript is a review of the literature concerning studies that compared in vitro-manipulated to in vivo-produced preimplantation embryos from the farm species. The authors apologize in advance for studies which were inadvertently omitted and acknowledge the diligent work of investigators who have published in this most challenging and important area of embryo development.

References (72)

  • Y Heyman et al.

    Developmental ability of bovine embryo after nuclear transfer based on the nuclear source: in vivo versus in vitro

    Theriogenology

    (1994)
  • P Holm et al.

    In vitro versus in vivo culture of ovine IVM-IVF ova: Effect on lambing

    Theriogenology

    (1994)
  • J Jura et al.

    In vitro and in vivo development of bovine embryos from zygotes and 2-cell embryos microinjected with exogenous DNA

    Theriogenology

    (1994)
  • RM Kunkel et al.

    Donor-recipient asynchrony, stage of embryo development and the post-transfer survival of bovine embryos

    Theriogenology

    (1978)
  • GM Lindner et al.

    Morphological evaluation of bovine embryos

    Theriogenology

    (1983)
  • DL Northey et al.

    Development of nuclear transfer embryos in in vivo versus in vitro culture systems

    Theriogenology

    (1992)
  • L Picard et al.

    Cytological studies of bovine half-embryos

    Theriogenology

    (1984)
  • JW Pollard et al.

    Comparative cryobiology of in vitro and in vivo derived bovine embryos

    Theriogenology

    (1993)
  • CE Rexroad et al.

    Co-culture of ovine eggs with oviductal cells and trophoblastic vesicles

    Theriogenology

    (1988)
  • JN Shelton et al.

    Survival of sheep DEMI-embryo in vivo and in vitro

    Theriogenology

    (1988)
  • A Van Soom et al.

    Compaction rate of in vitro fertilized bovine embryos related to the interval from insemination to first cleavage

    Theriogenology

    (1992)
  • SK Walker et al.

    In vitro culture of sheep embryos without co-culture: Successes and perspectives

    Theriogenology

    (1992)
  • SJ Warfield et al.

    Transfer of bovine demi-embryos with and without zonae pellucidae

    Theriogenology

    (1986)
  • JM Wright

    Non-surgical embryo transfer in cattle: embryo-recipient interactions

    Theriogenology

    (1981)
  • EC Amoroso et al.

    Early development of the goat

    J Anat

    (1942)
  • R Assheton

    The development of the pig during the first 10 days

    Quart J Micro Sci

    (1898)
  • NBA Ball et al.

    Viability of equine embryos co-cultured with equine oviductal epithelium from the 4–8 cell to blatocyst stage

    Theriogenology

    (1991)
  • FL Barnes et al.

    Embryo cloning in cattle: the use of in vitro matured oocytes

    J Reprod Fertil

    (1993)
  • KJ Betteridge et al.

    Development of horse embryos up to twenty-two days after ovulation: observations on fresh specimens

    J Anat

    (1982)
  • MC Chang

    Development of bovine blastocyst with a note on implantation

    Anat Rec

    (1952)
  • M Dorland et al.

    Rating bovine embryo quality by using a DNA-specific staining procedure

  • DF Dowling

    Problems of the transplantation of fertilized ova

    J Agricul Sci

    (1949)
  • JE Ellington et al.

    Bovine 1–2-cell embryo development using a semi-defined medium in three oviduct epithelial cell coculture systems

    Biol Reprod

    (1990)
  • JE Ellington et al.

    Development and survival after transfer of cow embryos cultured from 1–2-cells to morulae and blastocysts in rabbit oviducts or in a simple medium with bovine oviductal cells

    J Reprod Fertil

    (1990)
  • WH Eyestone et al.

    Co-culture of early cattle embryos to the blastocyst stage with oviductal tissue or in conditioned medium

    J Reprod Fert

    (1989)
  • PW Farin et al.

    Transfer of bovine embryos produced in vivo or in vitro: survival and fetal development

    Biol Reprod

    (1995)
  • Cited by (0)

    View full text