Elsevier

Theriogenology

Volume 52, Issue 5, 1 October 1999, Pages 835-846
Theriogenology

Contraceptive potential of the porcine zona pellucida vaccine in the African elephant (loxodonta africana)

https://doi.org/10.1016/S0093-691X(99)00176-4Get rights and content

Abstract

Immunocontraception has been successful in controlling free-roaming equids; however, what is the potential for the immunocontraceptive control of the African elephant (Loxodonta africana)? The porcine zona pellucida (pZP) glycoproteins share antigenic domains with the African elephant zona pellucida (elZP) glycoproteins, and anti-zona pellucida serum antibodies have been successfully stimulated. To determine the cross-reactivity of the pZP and elZP, immunocytochemistry was evaluated by light and electron microscopy. Specifically, the binding of polyclonal antibodies against total heat-solubilized-porcine zona pellucida to fixed elephant ovary sections was evaluated. The elZP of primary, secondary and tertiary follicles was recognized by the rabbit-anti-pZP serum, but there was no apparent recognition of the primordial follicles. The ability of anti-pZP antibodies to recognize the elZP demonstrates that there is molecular homology between the pZP and elZP glycoproteins. This homology makes the African elephant a candidate for pZP immunocontraception. Three captive elephants were vaccinated with 400 μg pZP with a synthetic trehalose dicorynomycolate (S-TDCM) adjuvant. The elephants received 2 boosters of 600 μg pZP at 4 wk and 10 mo after the primary vaccination. The vaccinated female elephants developed significant (P<0.05) titers to pZP over prevaccination levels. These levels persisted for 12 to 14 mo after the third vaccination. This preliminary evidence shows that the female elephant can develop significant serum antibody levels to pZP. These levels of antibodies are comparable to those required in horses for successful immunocontraception. Thus, porcine zona pellucida immunocontraception might be used to control elephant populations.

References (25)

  • LeveilleMC et al.

    Uptake of an oviductal antigen by the hamster zona pellucida

    Biol Reprod

    (1987)
  • LiuIKM et al.

    Contraception in mares heteroimmunized with pig zonae pellucidae

    J Reprod Fertil

    (1989)
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