Redox status of equine seminal plasma reflects the pattern and magnitude of DNA damage in sperm cells

Wnuk, Maciej; Lewinska, Anna; Oklejewicz, Bernadetta; Bartosz, Grzegorz; Tischner, Marek; Bugno-Poniewierska, Monika

doi:10.1016/j.theriogenology.2010.07.007

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Volume 74, Issue 9 , Pages 1677-1684, December 2010

Redox status of equine seminal plasma reflects the pattern and magnitude of DNA damage in sperm cells

Maciej Wnuk
- Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland
- Corresponding author. Tel.: +48178723711, fax: + 48178723711
Anna Lewinska
- Department of Biochemistry and Cell Biology, University of Rzeszow, Pigonia 6, 35-959 Rzeszow, Poland
Bernadetta Oklejewicz
- Department of Genetics, University of Rzeszow, Rejtana 16C, 35-959 Rzeszow, Poland
Grzegorz Bartosz
- Department of Biochemistry and Cell Biology, University of Rzeszow, Pigonia 6, 35-959 Rzeszow, Poland
Marek Tischner
- Department of Animal Reproduction and Anatomy, University of Agriculture, Mickiewicza 24/28, 30-059 Cracow, Poland
Monika Bugno-Poniewierska
- Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, Krakowska 1, Balice n. Cracow, Poland

Received 12 April 2010; received in revised form 3 July 2010; accepted 3 July 2010. published online 23 August 2010.

Abstract

Antioxidant status of seminal plasma from 23 stallions was evaluated. We found a negative correlation between total antioxidant capacity (ABTS^•+ decolorization assay) and thiol content of seminal plasma, and sperm DNA damage (8-oxoG immunostaining, TUNEL reaction, comet assay). Low seminal redox status was the strongest correlated with 8-oxoG level which may indicate that seminal total antioxidant capacity influences mainly the formation of single strand DNA breaks in sperm cells. Since inter-individual differences in seminal antioxidant status were reported, we postulated that the redox status of seminal plasma may be an additional important parameter, both with sperm quantitative and morphological analysis, for evaluation of equine semen quality.

Keywords: Semen, Spermatozoa, Equine, Total antioxidant capacity, DNA damage