Theriogenology
Volume 74, Issue 8 , Pages 1476-1481 , November 2010

Effect of extender supplementation with various antimicrobial agents on viability of Brucella ovis and Actinobacillus seminis in cryopreserved ovine semen

Received 22 January 2010 ,Revised 12 June 2010 ,Accepted 19 June 2010.

References 

  1. Burgess GW. Ovine contagious epididymitis: a review. Vet Microbiol. 1982;7:551–575
  2. Xavier MN, Costa EA, Paixão TA, Santos RL. The genus Brucella and clinical manifestations of brucellosis. Ciência Rural. 2009;39:2252–2260
  3. Thibier M, Guerin B. Hygienic aspects of storage and use of semen for artificial insemination. Anim Reprod Sci. 2000;62:233–251
  4. Althouse GC, Pierdon MS, Lu KG. Thermotemporal dynamics of contaminant bacteria and antimicrobials in extended porcine semen. Theriogenology. 2008;70:1317–1323
  5. Myers RM, Almquist JO. A comparison of the effects of aureomycin, penicillin and streptomycin upon spermatozoan livability and control of bacteria in bovine semen. J Anim Sci. 1951;10:322–330
  6. Akhter S, Ansari MS, Andrabi SMH, Ullah N, Qayyum M. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod Domest Anim. 2008;43:272–278
  7. Price S, Aurich J, Davies-Morel M, Aurich C. Effects of oxygen exposure and gentamicin on stallion semen stored at 5°C and 15°C. Reprod Domest Anim. 2008;43:261–266
  8. Kucers A, Bennet NMcK. Antibiotics. In: The Use of Antibiotics. JB Lippincott Company; 1975;
  9. Tsolis RM, Seshadri R, Santos RL, Sangari FJ, Lobo JM, de Jong MF, et al. Genome degradation in Brucella ovis corresponds with narrowing of its host range and tissue tropism. PLoS One. 2009;4:e5519
  10. Hajtos I, Fodor L, Glavits R, Varga J. Isolation and characterization of Actinobacillus seminis strains from ovine semen samples and epididymitis. J Vet Med. 1987;34:138–147
  11. Bulgin MS. Epididymitis in rams and lambs. Vet Clin North Am Food Anim Pract. 1990;6:683–690
  12. Grilló MJ, Marín CM, Barberán M, Blasco JM. Experimental Brucella ovis infection in pregnant ewes. Vet Rec. 1999;144:555–558
  13. Menzies PI, Miller R. Abortion in sheep: diagnosis and control. In:  Youngquist RS editors. Current Therapy in Large Animal Theriogenology. WB Saunders; 1999;
  14. Foster G, Collins MD, Lawson PA, Buxton D, Murray FJ, Sime A. Actinobacillus seminis as a cause of abortion in a UK sheep flock. Vet Rec. 1999;144:479–480
  15. Hajtós I. Isolation of Actinobacillus seminis and Histophilus ovis strains from aborted ovine fetuses in Hungary. Acta Vet Hung. 1987;35:415–425
  16. Appuhamy S, Low JC, Parton R, Coote JG. Specific PCR primers from the 16S-23S rRNA spacer region for the rapid detection and identification of Actinobacillus seminis. J Appl Microbiol. 1998;85:941–948
  17. Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the Brucellosis Laboratory. Paris: INRA; 1988;
  18. Harrison RAP, Vickers SE. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil. 1990;88:343–352
  19. Salamon S, Visser D. Effect of composition of tris-based diluent and of thawing solution on survival of ram spermatozoa frozen by the pellet method. Aust J Biol Sci. 1972;25:605–618
  20. Varner DD, Scanlan CM, Thompson JA, Brumbaugh GW, Blanchard TL, Carlton CM, et al. Bacteriology of preserved stallion semen and antibiotics in semen extenders. Theriogenology. 1998;50:559–573
  21. Rocha A, Oliveira E, Vilhena MJ, Diaz J, Sousa M. A novel apical midpiece defect in the spermatozoa of a bull without an apparent decrease in motility and fertility a case study. Theriogenology. 2006;66:913–922
  22. Baykam N, Esener H, Ergonul O, Eren S, Celikbas AK, Dokuzoguz B. In vitro antimicrobial susceptibility of Brucella species. Intern J Antimicrob Agents. 2004;23:405–407
  23. Price S, Aurich J, Davies-Morel M, Aurich C. Effects of oxygen exposure and gentamicin on stallion semen stored at 5 and 15 degrees C. Reprod Domest Anim. 2008;43:261–266
  24. Akhter S, Ansari MS, Andrabi SM, Ullah N, Qayyum M. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen. Reprod Domest Anim. 2008;43:272–278
  25. Guérin B, Thibier M. Value of adding antibiotics to frozen bovine semen: the example of mycoplasmas and campylobacters. Contracept Fertil Sex. 1993;21:753–759
  26. Souza AF, Souza MMP, Guerra ZF, Coleto RA, Mota LBG, Silva AEDS, et al. Avaliação microbiológica do sêmen fresco e congelado de reprodutores caprinos. Braz. J Vet Res Anim Sci. 2006;43:329–336

PII: S0093-691X(10)00337-7

doi: 10.1016/j.theriogenology.2010.06.019

Theriogenology
Volume 74, Issue 8 , Pages 1476-1481 , November 2010