Theriogenology
Volume 74, Issue 8 , Pages 1431-1438 , November 2010

Directional freezing as an alternative method for cryopreserving rhesus macaque (Macaca mulatta) sperm

  • Wei Si

      Affiliations

    • Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China
  • ,
  • Yongqing Lu

      Affiliations

    • Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China
  • ,
  • Xiechao He

      Affiliations

    • Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China
  • ,
  • Shaohui Ji

      Affiliations

    • Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China
  • ,
  • Yuyu Niu

      Affiliations

    • Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China
  • ,
  • Tao Tan

      Affiliations

    • Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China
  • ,
  • Weizhi Ji

      Affiliations

    • Kunming Institute of Zoology and Kunming Primate Research Center, Chinese Academy of Sciences, Yunnan Key Laboratory for Animal Reproduction, Kunming, Yunnan 650223, People's Republic of China
    • Kunming Biomed International, Kunming, Yunnan 650500, People's Republic of China
    • Corresponding Author InformationCorresponding author. Tel.: +86 871 5139413; fax: +86 871 5139413

Received 26 November 2009 ,Revised 15 June 2010 ,Accepted 15 June 2010.

References 

  1. Yang SH, Cheng PH, Banta H, Piotrowska-Nitsche K, Yang JJ, Cheng EC, et al. Towards a transgenic model of Huntington's disease in a non-human primate. Nature. 2008;453:921–924
  2. Chan AW, Chong KY, Martinovich C, Simerly C, Schatten G. Transgenic monkeys produced by retroviral gene transfer into mature oocytes. Science. 2001;291:309–312
  3. Si W, Zheng P, Tang X, He X, Wang H, Bavister BD, et al. Cryopreservation of rhesus macaque (Macaca mulatta) spermatozoa and their functional assessment by in vitro fertilization. Cryobiology. 2000;41:232–240
  4. Roussel JD, Austin CR. Preservation of primate spermatozoa by freezing. J Reprod Fertil. 1967;13:333–335
  5. Nichols SM, Bavister BD. Comparison of protocols for cryopreservation of rhesus monkey spermatozoa by post-thaw motility recovery and hyperactivation. Reprod Fertil Dev. 2006;18:777–780
  6. Leverage WE, Valerio DA, Schultz AP, Kingsbury E, Dorey C. Comparative study on the freeze preservation of spermatozoa (Primate, bovine, and human). Lab Anim Sci. 1972;22:882–889
  7. Leibo SP, Kubisch HM, Schramm RD, Harrison RM, VandeVoort CA. Male-to-male differences in post-thaw motility of rhesus spermatozoa after cryopreservation of replicate ejaculates. J Med Primatol. 2007;36:151–163
  8. Gagliardi C, Myers L, Kubisch HM. Postthaw survival of rhesus macaque sperm: variation in the response of individual males to different freezing protocols. Am J Primatol. 2008;70:1093–1096
  9. Gabriel Sanchez-Partida L, Maginnis G, Dominko T, Martinovich C, McVay B, Fanton J, et al. Live rhesus offspring by artificial insemination using fresh sperm and cryopreserved sperm. Biol Reprod. 2000;63:1092–1097
  10. Dong Q, Rodenburg SE, Huang C, VandeVoort CA. Effect of pre-freezing conditions on semen cryopreservation in rhesus monkeys. Theriogenology. 2008;70:61–69
  11. Watson PF. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci. 2000;60–61481–92
  12. Si W, Wang H, Reid C, Hildebrandt TB, Ji W. Effect of sugar type on the survival of frozen-thawed rhesus monkey (Macaca mulatta) sperm. Am J Primatol. 2006;68:103–108
  13. Stacy R, Eroglu A, Fowler A, Biggers J, Toner M. Thermal characterization of Nakagata's mouse sperm freezing protocol. Cryobiology. 2006;52:99–107
  14. Koshimoto C, Mazur P. Effects of cooling and warming rate to and from −70 degrees C, and effect of further cooling from −70 to −196 degrees C on the motility of mouse spermatozoa. Biol Reprod. 2002;66:1477–1484
  15. Si W, Hildebrandt TB, Reid C, Krieg R, Ji W, Fassbender M, et al. The successful double cryopreservation of rabbit (Oryctolagus cuniculus) semen in large volume using the directional freezing technique with reduced concentration of cryoprotectant. Theriogenology. 2006;65:788–798
  16. Saragusty J, Hildebrandt TB, Behr B, Knieriem A, Kruse J, Hermes R. Successful cryopreservation of Asian elephant (Elephas maximus) spermatozoa. Anim Reprod Sci. 2009;115:255–266
  17. Saragusty J, Gacitua H, Pettit MT, Arav A. Directional freezing of equine semen in large volumes. Reprod Domest Anim. 2007;42:610–615
  18. Saragusty J, Gacitua H, King R, Arav A. Post-mortem semen cryopreservation and characterization in two different endangered gazelle species (Gazella gazella and Gazella dorcas) and one subspecies (Gazella gazelle acaiae). Theriogenology. 2006;66:775–784
  19. Hermes R, Goritz F, Saragusty J, Sos E, Molnar V, Reid CE, et al. First successful artificial insemination with frozen-thawed semen in rhinoceros. Theriogenology. 2009;71:393–399
  20. Arav A, Zeron Y, Shturman H, Gacitua H. Successful pregnancies in cows following double freezing of a large volume of semen. Reprod Nutr Dev. 2002;42:583–586
  21. Gacitua H, Arav A. Successful pregnancies with directional freezing of large volume buck semen. Theriogenology. 2005;63:931–938
  22. Arav A. Device and methods for multigradient directional cooling and warming of biological samples. US Patent 1999;5 873 254
  23. Sankai T, Terao K, Yanagimachi R, Cho F, Yoshikawa Y. Cryopreservation of spermatozoa from cynomolgus monkeys (Macaca fascicularis). J Reprod Fertil. 1994;101:273–278
  24. Gould KG, Mann DR. Comparison of electrostimulation methods for semen recovery in the rhesus monkey (Macaca mulatta). J Med Primatol. 1988;17:95–103
  25. Bavister BD, Boatman DE, Leibfried L, Loose M, Vernon MW. Fertilization and cleavage of rhesus monkey oocytes in vitro. Biol Reprod. 1983;28:983–999
  26. Varisli O, Uguz C, Agca C, Agca Y. Motility and acrosomal integrity comparisons between electro-ejaculated and epididymal ram sperm after exposure to a range of anisosmotic solutions, cryoprotective agents and low temperatures. Anim Reprod Sci. 2009;110:256–268
  27. Yang S, He X, Hildebrandt TB, Jewgenow K, Goeritz F, Tang X, et al. Effects of rhFSH dose on ovarian follicular response, oocyte recovery and embryo development in rhesus monkeys. Theriogenology. 2007;67:1194–1201
  28. Liu Z, Foote RH, Brockett CC. Survival of bull sperm frozen at different rates in media varying in osmolarity. Cryobiology. 1998;37:219–230
  29. Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol. 1984;247:C125–C142
  30. Morris GJ. Rapidly cooled human sperm: no evidence of intracellular ice formation. Hum Reprod. 2006;21:2075–2083
  31. Hernandez M, Ekwall H, Roca J, Vazquez JM, Martinez E, Rodriguez-Martinez H. Cryo-scanning electron microscopy (Cryo-SEM) of semen frozen in medium-straws from good and sub-standard freezer AI-boars. Cryobiology. 2007;54:63–70
  32. Dong Q, Hill D, VandeVoort CA. Interactions among pre-cooling, cryoprotectant, cooling, and thawing for sperm cryopreservation in rhesus monkeys. Cryobiology. 2009;59:268–274
  33. Si W, Zheng P, Li Y, Dinnyes A, Ji W. Effect of glycerol and dimethyl sulfoxide on cryopreservation of rhesus monkey (Macaca mulatta) sperm. Am J Primatol. 2004;62:301–306
  34. Schramm RD, Bavister BD. Development of in-vitro-fertilized primate embryos into blastocysts in a chemically defined, protein-free culture medium. Hum Reprod. 1996;11:1690–1697

PII: S0093-691X(10)00333-X

doi: 10.1016/j.theriogenology.2010.06.015

Theriogenology
Volume 74, Issue 8 , Pages 1431-1438 , November 2010