Theriogenology
Volume 74, Issue 7 , Pages 1264-1270, 15 October 2010

Large scale in vivo risk assessment of bovine viral diarrhea virus (BVDV) transmission through transfer of bovine embryos produced via somatic cell nuclear transfer (SCNT)

  • K. Gregg

      Affiliations

    • Viagen, Inc., 12357-A Riata Trace Parkway, Suite 100, Austin, TX 78727
    • Corresponding Author InformationCorresponding author. Tel.: 512-401-5903; fax: 512-401-5919
    • ,
  • G. Gosch

      Affiliations

    • Trans Ova Genetics, 2938 380th St, Sioux Center, IA 51250
    • ,
  • T. Guerra

      Affiliations

    • Viagen, Inc., 12357-A Riata Trace Parkway, Suite 100, Austin, TX 78727
    • ,
  • S.H. Chen

      Affiliations

    • Viagen, Inc., 12357-A Riata Trace Parkway, Suite 100, Austin, TX 78727
    • ,
  • T. Xiang

      Affiliations

    • Viagen, Inc., 12357-A Riata Trace Parkway, Suite 100, Austin, TX 78727
    • ,
  • D. Broek

      Affiliations

    • Trans Ova Genetics, 2938 380th St, Sioux Center, IA 51250
    • ,
  • B. Bruner

      Affiliations

    • Viagen, Inc., 12357-A Riata Trace Parkway, Suite 100, Austin, TX 78727
    • ,
  • I. Polejaeva

      Affiliations

    • Viagen, Inc., 12357-A Riata Trace Parkway, Suite 100, Austin, TX 78727

Received 14 April 2010; received in revised form 24 May 2010; accepted 29 May 2010. published online 13 August 2010.

Abstract 

The objective was to use the bovine viral diarrhea virus (BVDV) as a model to assess the risk of infectious disease transmission in the system of in vitro embryo production and transfer via somatic cell nuclear transfer (SCNT) technology. The risks of BVDV transmission in the SCNT embryo production were previously evaluated [1]. In that in vitro study, following standard operating procedures (SOP), including pre-nuclear transfer donor cell testing, oocyte decontamination and virus-free cell and embryo culture conditions, SCNT embryos produced were free of detectable viral RNA. The current study focused on the evaluation of the potential risk of disease transmission from SCNT embryos to recipients, and the risk of producing persistently infected animals via SCNT embryo transfer. Blood samples were collected from 553 recipients of SCNT embryos and 438 cloned calves and tested for the presence of BVDV viral RNA via a sensitive real time PCR method. All samples tested were negative. These results, in conjunction with the previous in vitro study, confirmed that the established SCNT embryo production and transfer system is safe and presents no detectable risk of disease transmission.

Keywords: BVDV transmission, Risk Assessment, Somatic Cell Nuclear Transfer (SCNT), Embryo, Recipient

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PII: S0093-691X(10)00300-6

doi:10.1016/j.theriogenology.2010.05.032

Theriogenology
Volume 74, Issue 7 , Pages 1264-1270, 15 October 2010

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