Theriogenology
Volume 74, Issue 1 , Pages 153-164 , 1 July 2010

Effect of post-thaw dilution with caffeine, pentoxifylline, 2’-deoxyadenosine and prostatic fluid on motility of frozen-thawed dog semen

  • C. Milani

      Affiliations

    • Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Agripolis, 35020 Legnaro- PD (Italy)
    • Corresponding Author InformationCorresponding author. Tel.: +39 049 8272947; fax: +39 049 8272954.
    • ,
  • A. Fontbonne

      Affiliations

    • C.E.R.C.A- Alfort National Veterinary College, 7, avenue du général de Gaulle, 94700 Maisons-Alfort cedex- France
    • ,
  • E. Sellem

      Affiliations

    • U.N.C.E.I.A- Département R&D,13 rue Jouët, 94704 Maisons-Alfort cedex- France
    • ,
  • C. Stelletta

      Affiliations

    • Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Agripolis, 35020 Legnaro- PD (Italy)
    • ,
  • O. Gérard

      Affiliations

    • U.N.C.E.I.A- Département R&D,13 rue Jouët, 94704 Maisons-Alfort cedex- France
    • ,
  • S. Romagnoli

      Affiliations

    • Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Agripolis, 35020 Legnaro- PD (Italy)

Received 6 November 2009 ,Revised 14 January 2010 ,Accepted 31 January 2010.

References 

  1. Rota A, Peña AI, Linde-Forsberg , Rodriguez-Martinez H. In vitro capacitation of fresh, chilled and frozen-thawed dog spermatozoa assessed by the chloretetracycline assay and changes in motility patterns. Anim Reprod Sci. 1999;57:199–215
  2. Dobrinsky I, Lulai C, Barth AD, Post K. Effect of four different extenders and three different freezing rates on post-thaw viability of dog semen. J Reprod Fertil (Suppl). 1993;47:291–296
  3. Rota A, Frishling A, Vannozzi I, Camillo F, Romagnoli S. Effect of inclusion of skimmed milk in freezing extenders on the viability of canine spermatozoa after thawing. J Reprod Fert (Suppl). 2001;57:377–381
  4. Cardoso R, Silva AR, Silva LDM, Chirinéa VH, Souza FF, Lopes MD. Evaluation of fertilizing potential of frozen-.thawed dog spermatozoa diluted in ACP-106® using in vitro sperm-oocyte interaction assay. Reprod Dom Anim. 2007;42:11–16
  5. Fontbonne A, Badinand F. Studies on freezing dog spermatozoa: effect of glycerol on motility after thawing. J Reprod Fertil (Suppl). 1993;47:531–532
  6. Silva AR, Cardoso RC, Uchoa DC, Silva LD. Quality of canine semen submitted to single or fractionated glycerol addition during the freezing process. Theriogenology. 2003;59:821–829
  7. Okano T, Murase T, Asano M, Tsubota T. Effects of final dilution rate, sperm concentration and times for cooling and glycerol equilibration on post-thaw characteristics of canine spermatozoa. J Vet Med Sci. 2004;66:1359–1364
  8. Rota A, Milani C, Cabianca G, Martini M. Comparison between glycerol and ethylene glycol for dog semen cryopreservation. Theriogenology. 2006;65:1848–1858
  9. Rota A, Linde-Forsberg C, Vannozzi J, Romagnoli S, Rodriguez-Martinez H. Cryosurvival of dog spermatozoa at different glycerol concentrations and freezing/thawing rates. Reprod Dom Anim. 1998;33:355–361
  10. Peña AI, Linde-Forsberg C. Effects of Equex, one or two step dilution and two freezing and thawing rates on post-thaw survival of dog spermatozoa. Theriogenology 2000(a);54:859-75.
  11. Rota A, Rota A, Martini M, Milani C, Romagnoli S. Evaluation of dog semen quality after slow (biological freezer) or rapid (nitrogen vapours) freezing. Reprod Nutr Dev. 2005;45:29–37
  12. Batista M, Alamo D, Gonzàlez F, Cruz MG, Gracia A. Influence of the freezing technique (Nitrogen Liquid vs Ultrafreezer of -152°C) and male-to-male variation over the semen quality in Canarian Mastiff breed dogs. Reprod Dom Anim. 2006;41:423–428
  13. Schäfer-Somi S, Kluger S, Knapp E, Klein D, Aurich C. Effects of semen extender and semen processing on motility and viability of frozen-thawed dog spermatozoa. Theriogenology. 2006;15:173–182
  14. Nöthling JO, Shuttleworth R. The effect of straw size, freezing rate and thawing rate upon post-thaw quality of dog semen. Theriogenology. 2005;63:1469–1480
  15. Nöthling JO, Dolieslager SM, Fillekes R, Colenbrander B. Thawing dog spermatozoa in just-boiled water: submersion time and effect on sperm quality compared to thawing in water at 70 degrees C. Theriogenology. 2007;68:530–537
  16. Yu I, Songsasen N, Godke RA, Leibo SP. Differences among dogs in response of their spermatozoa to cryopreservation using various cooling and warming rates. Cryobiology. 2002;44:62–78
  17. Andersen K. Insemination with frozen dog semen based on a new insemination technique. Zuchthygiene. 1975;10:1–4
  18. Tsutsui T, Hase M, Hori T, Komoriya K, Shimizu N, Nagakubo K, et al. Effect of addition of Orvus ES Paste® to frozen canine semen extender on sperm acrosomes. J Vet Med Sci. 2000;62:537–538
  19. Rota A, Ström B, Linde-Forsberg C, Rodriguez-Martinez H. Effect of Equex STM Paste on viability of frozen-thawed dog spermatozoa during in vitro incubation at 38°C. Theriogenology. 1997;47:1093–1101
  20. Peña AI, Linde-Forsberg C. Effects of spermatozoal concentration and post-thaw dilution rate on survival after thawing of dog spermatozoa. Theriogenology 2000(b);54:703-18.
  21. Harrison RF. Insemination of husband's semen with and without the addition of caffeine. Fertil Steril. 1978;29:532–534
  22. Sharma RK, Tolentino MV, Agarwal A. Sperm kinematics of cryopreserved normozoospermic specimens after artificial stimulation. Urology. 1996;47:77–81
  23. Jayaprakash D, Santosh Kumar K, Shivaji S, Seshagiri PB. Pentoxifylline induces hyperactivation and acrosome reaction in spermatozoa of golden hamsters: changes in motility kinematics. Hum Reprod. 1997;12:2192–2199
  24. Sinha MP, Sinha AK, Singh BK. Effect of methylxanthines on motility and fertility of frozen-thawed goat semen. Theriogenology. 1995;44:907–914
  25. Funahashi H, Nagai T. Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine. Mol Reprod Dev. 2001;58:424–431
  26. Stachecki JJ, Dresser BL, Pope CE, Armant DR. Stimulation of ejaculated domestic cat sperm motility with caffeine, pentoxifylline, and 2’-deoxyadenosine. Arch Androl. 1995;34:63–68
  27. Vijayaraghavan S, Hoskins D. Regulation of bovine sperm motility and cyclic adenosine 3’, 5’-monophosphate by adenosine and its analogue. Biol Reprod. 1986;34:468–477
  28. Gradil CM, Ball BA. The use of pentoxifylline to improve motility of cryopreserved equine spermatozoa. Theriogenology. 2000;54:1041–1047
  29. Koutsarova N, Todorov P, Koutsarov G. Effect of pentoxifylline on motility and longevity of fresh and thawed dog spermatozoa. J Reprod Fertil (Suppl). 1997;51:117–121
  30. Nöthling JO, Volkmann DH. Effect of addition of autologous prostatic fluid on the fertility of frozen-thawed dog semen after intravaginal insemination. J Reprod Fertil (Suppl). 1993;47:329–333
  31. Rota A, Milani C, Romagnoli S. Effect of post-thaw dilution with autologous prostatic fluid on dog semen motility and sperm acrosome status. Theriogenology. 2007;67:520–525
  32. Iguer-Ouada M, Verstegen JP. Validation of Sperm Quality Analyzer (SQA), for dog sperm analysis. Theriogenology 2001(a);55:1143-58.
  33. Iguer-Ouada M, Verstegen JP. Evaluation of the “Hamilton Thorn computer-based automated system” for dog semen analysis. Theriogenology 2001(b);55:733-49.
  34. Schäfer-Somi S, Aurich C. Use of a new computer-assisted sperm analyzer for the assessment of motility and viability of dog spermatozoa and evaluation of four different semen extender for predilution. Anim Repro Sci. 2007;102:1–13
  35. Linde-Forsberg C. Hints on dog semen freezing, cryoextenders, and frozen semen artificial insemination. Proc. STF Colorado Springs. 2002;303–320
  36. Henkel RR, Schill WB. Sperm preparation for ART. Reprod Biol Endocrinol. 2003;14:108
  37. Tesarik J, Mendoza C. Sperm treatment with pentoxifylline improves the fertilizing ability in patients with acrosome reaction insufficiency. Fertil Steril. 1993;60:141–148
  38. Lanzafame F, Chapman MG, Guglielmino A, Gearon CM, Forman RG. Pharmacological stimulation of sperm motility. Hum Reprod. 1994;9:192–199
  39. Karakagil M, Imamoglu A, Pasaoglu H, Gulmez I, Tatlisen A. The effects of caffeine and theophylline on the triple adenosine triphosphatase enzyme activities of spermatozoa from males with oligoasthenospermia. Eur Urol. 1990;17:69–71
  40. Sharma RK, Agarwal A. Artificial stimulation of cryopreserved human spermatozoa by sodium nitroprusside, 2-chloroadenosine, and 2-deoxyadenosine. Eur Urol. 1997;32:344–352
  41. Numabe T, Oikawa T, Kikuchi T, Horiuchi T. Pentoxifylline improves in vitro fertilization and subsequent development of bovine oocytes. Theriogenology. 2001;56:225–233
  42. Stachecki JJ, Ginsburg KA, Armant DR. Stimulation of cryopreserved epididymal spermatozoa of the domestic cat using the motility stimulants caffeine, pentoxifylline, and 2’-deoxyadenosine. J Androl. 1994;15:157–164
  43. Imoedemhe DA, Sigue AB, Pacpaco EL, Olazo AB. The effect of caffeine on the ability of spermatozoa to fertilize mature human oocytes. J Assisted Reprod Genet. 1992;9:155–160
  44. Sirivaidyapong S, Ursem P, Bevers MM, Colenbrander B. Effect of prostatic fluid on motility, viability and acrosome integrity of chilled and frozen-thawed dog spermatozoa. J Reprod Fertil (Suppl). 2001;57:383–386
  45. Ribeiro APC, Vincente WRR, Apparicio M, Gadelha CRF, Alves AE, Covizzi GJ. Uterine leucocyte infiltration after artificial insemination in bitches. Theriogenology. 2006;66:1462–1464
  46. Suarez SS, Katz DF, Owen DH, Andrew JB, Powell RL. Evidence for the function of hyperactivated motility in sperm. Biol Reprod. 1991;44:375–381
  47. Guérin GF. Méthodes d’analyse de la mobilité des spermatozoides: intérêt dans un bilan de fertilité masculine. Contracep Fertil Sex 1991; 19:793-7.(In French, abstract in English).
  48. Jeulin C, Feneux D, Serres C, Jouannet P, Guillet-Rosso F, Bellaish-Alart J, et al. Sperm factors related to failure of human in vitro fertilization. J Reprod Fertil. 1986;76:735–744
  49. Silva AR, Cardoso RS, Silva LDM, Chrinéa VH, Lopes MD, Souza FF. Prognostic value of canine frozen-thawed semen parameters on in vitro sperm-oocyte interactions. Theriogenology. 2006;66:456–462
  50. Rigau T, Farré M, Ballester J, Mogas T, Peña A, Rodriguez-Gil JE. Effects of glucose and fructose on motility patterns of dog spermatozoa from fresh ejaculates. Theriogenology. 2001;56:801–815

PII: S0093-691X(10)00079-8

doi: 10.1016/j.theriogenology.2010.01.026

Theriogenology
Volume 74, Issue 1 , Pages 153-164 , 1 July 2010

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