Differentiation diversity of mouse parthenogenetic embryonic stem cells in chimeric mice
Received 19 September 2009; received in revised form 16 January 2010; accepted 30 January 2010. published online 08 March 2010.
Abstract
Recent studies have illustrated multiple differentiation potentials of embryonic stem cells (ESCs), derived from parthenogenetic embryos, to various kinds of cells (all three embryonic germ layers). However, differentiation diversity of the parthenogenetic ESCs (PgESCs) in vivo remains to be elucidated. In the present study, we established mouse PgESC-lines and observed their contribution diversity in vivo by producing chimeric mice using embryos possessing single nucleotide polymorphisms of mitochondrial DNA (mtDNA) as hosts. Based on southern blot analysis using specific probes to detect the SNPs on mtDNA, PgESC-derived mtDNA were contained in many organs such as brain, lung, and heart of the chimeric mouse. We concluded that PgESCs contributed to various internal organs in vivo, and that they were also stably maintained in adult animals.
aInstitute of Advanced Clinical Medicine, Kinki University School of Medicine, Ohno-Higashi, Osaka-Sayama, Osaka 589-8511, Japan
bDepartment of Genetic Engineering, Graduate School of Biology Oriented Science and Technology, Kinki University, 930 Nishimitani, Kinokawa, Wakayama 649-6493, Japan
cDepartment of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan
dInstitute of Advanced Technology, Kinki University, 14-1 Minamiakasaka, Kainan, Wakayama 642-0017, Japan
eDepartment of Orthopaedic Surgery, Kinki University School of Medicine, Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan
fDepartment of Rehabilitation Medicine, Kinki University School of Medicine, Ohno-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan