Theriogenology
Volume 74, Issue 1 , Pages 118-126 , 1 July 2010

Influence of different centrifugation protocols on equine semen preservation

Received 29 June 2009 ,Revised 18 January 2010 ,Accepted 31 January 2010.

References 

  1. Jasko DJ, Moran DM, Farlin ME, Squires EL. Effect of seminal plasma dilution or removal on spermatozoal motion characteristics of cooled stallion semen. Therio. 1991;35:1059–1067
  2. Martin JC, Klug E, Günzel A-R. Centrifugation of stallion semen and its storage in large volume straws. J Reprod Fert Suppl. 1979;27:47–51
  3. Ecot P, Decuadro-Hansen G, Delhomme G, Vidament M. Evaluation of a cushioned centrifugation technique for processing equine semen for freezing. Anim Reprod Sci. 2005;89:245–248
  4. Knop K, Hoffmann N, Rath D, Sieme H. Effects of cushioned centrifugation technique on sperm recovery and sperm quality in stallions with good and poor semen freezability. Anim Reprod Sci. 2005;89:294–297
  5. Vidament M, Ecot P, Noue P, Bourgeois C, Magistrini M, Palmer E. Centrifugation and addition of glycerol at 22 degrees C instead of 4 degrees C improve post-thaw motility and fertility of stallion spermatozoa. Therio. 2000;54:907–919
  6. Weiss S, Janett F, Burger D, Hässig M, Thun R. The influence of centrifugation on quality and freezability of stallion semen. Schweiz Arch Tierheilk. 2004;146:285–293
  7. Loomis PR. Advanced methods for handling and preparation of stallion semen. Vet Clin North Am Equine Pract. 2006;22:663–676
  8. Aurich C. Recent advances in cooled-semen technology. Anim Reprod Sci. 2008;107:268–275
  9. Shekarriz M, DeWire DM, Thomas AJ, Agarwal A. A method of human semen centrifugation to minimize the iatrogenic sperm injuries caused by reactive oxygen species. Eur Urol. 1995;28:31–35
  10. Carvajal G, Cuello C, Ruiz M, Vázquez JM, Martínez EA, Roca J. Effects of centrifugation before freezing on boar sperm cryosurvival. J Androl. 2004;25:389–396
  11. Heitland AV, Jasko DJ, Squires EL, Graham JK, Pickett BW, Hamilton C. Factors affecting motion characteristics of frozen-thawed stallion spermatozoa. Equine Vet J. 1996;28:47–53
  12. Picket BW. Collection and evaluation of stallion semen for artificial insemination. In:  McKinnon AO,  Voss JL editor. Equine Reproduction. Williams & Wilkins; 1993;p. 705–714
  13. Barth AD, Oko RJ. Abnormal morphology of bovine spermatozoa. Ames, Iowa: Iowa State University Press; 1989;
  14. Rijsselaere T, Van Soom A, Maes D, de Kruif A. Effect of technical settings on canine semen motility parameters measured by the Hamilton-Thorne analyzer. Therio. 2003;60:1553–1568
  15. Loomis PR, Graham JK. Commercial semen freezing: individual male variation in cryosurvival and the response of stallion sperm to customized freezing protocols. Anim Reprod Sci. 2008;105:119–128
  16. Garner DL, Johnson LA. Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod. 1995;53:276–284
  17. Rathi R, Colenbrander B, Stout TA, Bevers MM, Gadella BM. Progesterone induces acrosome reaction in stallion spermatozoa via a protein tyrosine kinase dependent pathway. Mol Reprod Dev. 2003;64:120–128
  18. De Pauw IMC, Van Soom A, Mintiens K, Verberckmoes S, de Kruif A. In vitro survival of bovine spermatozoa stored at room temperature under epididymal conditions. Therio. 2003;59:1093–1107
  19. Gil MC, García-Herreros M, Barón FJ, Aparicio IM, Santos AJ, García-Marín LJ. Morphometry of porcine spermatozoa and its functional significance in relation with the motility parameters in fresh semen. Therio. 2009;71:254–263
  20. Love CC, Brinsko SP, Rigby SL, Thompson JA, Blanchard TL, Varner DD. Relationship of seminal plasma level and extender type to sperm motility and DNA integrity. Therio. 2005;63:1584–1591
  21. Evans DP, Wixon R. Clinical aspects of sperm DNA fragmentation detection and male infertility. Therio. 2006;65:979–991
  22. Cochran JD, Amann RP, Froman DP, Pickett BW. Effects of centrifugation, glycerol level, cooling to 5°C, freezing rate and thawing rate on the post-thaw motility of equine sperm. Therio. 1984;22:25–38
  23. Revell SG, Pettit MT, Ford TC. Use of centrifugation over iodixanol to reduce damage when processing stallion sperm for freezing. J Reprod Fertil 1997, Abstr series no. 19, p38.
  24. Waite JA, Love CC, Brinsko SP, Teague SR, Salazar JL, Mancil SS, et al. Factors impacting equine sperm recovery rate and quality following cushioned centrifugation. Therio. 2008;70:704–714

PII: S0093-691X(10)00075-0

doi: 10.1016/j.theriogenology.2010.01.022

Theriogenology
Volume 74, Issue 1 , Pages 118-126 , 1 July 2010

Article Tools

* Image Usage & Resolution