Theriogenology
Volume 74, Issue 1 , Pages 31-39 , 1 July 2010

In vitro evaluation of fresh sperm quality in tomcats: A comparison of two collection techniques

  • M. Filliers

      Affiliations

    • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
    • Corresponding Author InformationCorresponding author. Tel.: +32 9 264 75 51; fax: +32 9 264 77 97.
    • ,
  • T. Rijsselaere

      Affiliations

    • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
    • ,
  • P. Bossaert

      Affiliations

    • Veterinary Clinical Department, Obstetric-Gynaecological Section, University of Bologna, Ozzano Emilia (BO), Italy
    • ,
  • D. Zambelli

      Affiliations

    • Veterinary Clinical Department, Obstetric-Gynaecological Section, University of Bologna, Ozzano Emilia (BO), Italy
    • ,
  • P. Anastasi

      Affiliations

    • Veterinary Clinical Sciences, Obstetrics and Gynaecology, Università degli Studi di Milano, Milano, Italy
    • ,
  • M. Hoogewijs

      Affiliations

    • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium
    • ,
  • A. Van Soom

      Affiliations

    • Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

Received 7 August 2009 ,Revised 7 December 2009 ,Accepted 13 December 2009.

References 

  1. Tsutsui T. Artificial insemination in domestic cats (Felis catus). Theriogenology. 2006;66:122–125
  2. Pope CE, Keller GL, Dresser BL. In vitro fertilization in domestic and non-domestic cats including sequences of early nuclear events, development in vitro, cryopreservation and successful intra- and interspecies embryo transfer. J Reprod Fertil Suppl. 1993;47:189–201
  3. Pope CE. In vitro fertilization and embryo transfer in felids. In: Methods in Molecular Biology—germ cell protocols, volume 2: molecular embryo analysis, live imaging, transgenesis and cloning, Schatten H (Ed.), The Humana Press Inc., Totowa NJ, 2004, pp. 227-44.
  4. Platz CC, Seager SW. Semen collection by electroejaculation in the domestic cat. J Am Vet Med Assoc. 1978;173:1353–1355
  5. Zambelli D, Merlo B, Iacono E, Prati F, Belluzzi S. Fertilizing ability of electro-ejaculated cryopreserved semen in the domestic cat. Reprod Domest Anim. 2006;41:137–141
  6. Tanaka A, Takagi Y, Nakagawa K, Fujimoto Y, Hori T, Tsutsui T. Artificial intravaginal insemination using fresh semen in cats. J Vet Med Sci. 2000;62:1163–1167
  7. Zambelli D, Cunto M. Semen collection in cats: techniques and analysis. Theriogenology. 2006;66:159–165
  8. Bowen RA. Fertilization in vitro of feline ova by spermatozoa from the ductus deferens. Biol Reprod. 1977;17:144–147
  9. Hay MA, Goodrowe KL. Comparative cryopreservation and capacitation of spermatozoa from epididymis and vasa deferentia of the domestic cat. J Reprod Fertil Suppl. 1993;47:297–305
  10. Lengwinat T, Blottner S. In vitro fertilization of follicular oocytes of domestic cat using fresh and cryopreserved epididymal spermatozoa. Anim Reprod Sci. 1994;35:291–301
  11. Axnér E. Collection of epididymal spermatozoa. In: Proceedings, Reproduction in companion, exotic and laboratory animals III, course. Recent advances in reproduction. 13-18 September 2004, School of Veterinary Medicine, Milan, Italy, p. 12–4.
  12. Filliers M, Rijsselaere T, Bossaert P, De Causmaecker V, Dewulf J, Pope CE, et al. Computer-assisted sperm analysis of fresh epididymal cat spermatozoa and the impact of cool storage (4 degrees C) on sperm quality. Theriogenology. 2008;70:1550–1559
  13. Zambelli D, Prati F, Cunto M, Iacono E, Merlo B. Quality and in vitro fertilizing ability of cryopreserved cat spermatozoa obtained by urethral catheterization after medetomidine administration. Theriogenology. 2008;69:485–490
  14. Axnér E, Ström Holst B, Linde-Forsberg C. Morphology op spermatozoa in the cauda epididymidis before and after electroejaculation and a comparison with ejaculated spermatozoa in the domestic cat. Theriogenology. 1998;50:973–979
  15. Tebet JM, Martins MIM, Chirinea VH, Souza FF, Campagnol D, Lopes MD. Cryopreservation effects on domestic cat epididymal versus electroejaculated spermatozoa. Theriogenology. 2006;66:1629–1632
  16. Hermansson U, Axnér E. Epididymal and ejaculated cat spermatozoa are resistant to cold shock but egg yolk promotes sperm longevity during cold storage at 4°C. Theriogenology. 2007;67:1239–1248
  17. Mocé E, Graham JK. In vitro evaluation of sperm quality. Anim Reprod Sci. 2008;105:104–118
  18. Axnér E, Linde-Forsberg C. Sperm morphology in the domestic cat, and its relation with fertility: A retrospective study. Reprod Dom Anim. 2007;42:282–291
  19. Wood TC, Wildt DE. Effect of the quality of the cumulus–oocyte complex in the domestic cat on the ability of oocytes to mature, fertilize and develop into blastocysts in vitro. J Reprod Fertil. 1997;110:355–436
  20. Goodrowe KL, Howard JG, Schmidt PM, Wildt DE. The reproductive biology of the domestic cat with special reference to endocrinology, sperm function and in vitro fertilization. J Reprod Fertil. 1989;39:73–90
  21. Howard JG, Brown JL, Bush M, Wildt DE. Teratospermic and normospermic domestic cats: ejaculate traits, pituitary-gonadal hormones, and improvement of spermatozoal motility and morphology after swim-up processing. J Androl. 1990;11:204–215
  22. Harris RF, Gomez MF, Leibo SP, Pope CE. In vitro development of domestic cat embryos after in vitro fertilization of oocytes with spermatozoa stored for various intervals at 4°C. Theriogenology 2002; 57:365.(Abstract).
  23. Baas JW, Molan PC, Shannon P. Factors in seminal plasma of bulls that affect the viability and motility of spermatozoa. J Reprod Fert. 1983;68:275–280
  24. Goovaerts IGF, Hoflack GG, Van Soom A, Dewulf J, Nichi M, de Kruif A, et al. Evaluation of epididymal semen quality using the Hamilton–Thorne analyser indicates variation between the two caudae epididymides of the same bull. Theriogenology. 2006;66:323–330
  25. Ellington J, Scarlett J, Meyers-Wallen V, Mohammed HO, Surman V. Computer-assisted sperm analysis of canine spermatozoa motility measurements. Theriogenology. 1993;40:725–733
  26. Axnér E, Ström B, Linde-Forsberg C. Sperm morphology is better in the second ejaculate than in the first in domestic cats electroejaculated twice during the same period of anesthesia. Theriogenology. 1997;47:929–934
  27. Griggers S, Paccamonti DL, Thompson RA, Eilts BE. The effects of pH, osmolarity and urine contamination on equine spermatozoa motility. Theriogenology. 2001;56:613–622
  28. Okano T, Murase T, Nakamura S, Komatsu T, Tsubota T, Asano M. Morphological Spermatozoa among Peri-mating Seasons in Captive Japanese Black Bears (Ursus thibetanus japonicus). J Reprod Dev. 2009;55:194–199
  29. Peña AI, Barrio M, Becerra JJ, Quintela LA, Herradón PG. Infertility in a dog due to proximal cytoplasmic droplets in the ejaculate: investigation of the significance for sperm functionality in vitro. Reprod Domest Anim. 2007;42:471–478
  30. Söderquist L, Janson L, Larsson K, Einarsson S. Sperm morphology and fertility in A.I. bulls. J Vet Med A. 1991;38:534–543
  31. Oettlé EE. Sperm morphology and fertility in the dog. J Reprod Fertil Suppl. 1993;47:257–260
  32. Aitken RJ, Clarkson JS. Significance of reactive oxygen species and antioxidants in defining the efficiency of sperm preparation techniques. J Androl. 1988;9:367–376
  33. Thundathil J, Palasz AT, Barth AD, Mapletoft RJ. The use of in vitro fertilization techniques to investigate the fertilizing ability of bovine sperm with proximal droplets. Anim Reprod Sci. 2001;65:181–192
  34. Rao AR, Bane A, Gustafsson BK. Changes in the morphology of spermatozoa during their passage through the genital tract in dairy bulls with normal and impaired spermatogenesis. Theriogenology. 1980;14:1–12
  35. De Pauw IMC, Van Soom A, Mintiens K, Verberckmoes S, de Kruif A. In vitro survival of bovine spermatozoa stored at room temperature under epididymal conditions. Theriogenology. 2003;59:1093–1107
  36. Pintado B, de la Fuente J, Roldan ER. Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechst 33258, or to eosin: accuracy in the assessment of cell viability. J Reprod Fertil. 2000;118:145–152
  37. Pukazhenthi B, Pelican K, Wildt D, Howard J. Sensitivity of domestic cat (Felis catus) sperm from normospermic versus teratospermic donors to cold-induced acrosomal damage. Biol Reprod. 1999;61:135–141
  38. Fraser LR, Adeoya-Osiguwa SA, Baxendale RW, Gibbons R. Regulation of mammalian sperm capacitation by endogenous molecules. Front Biosci. 2006;11:1636–1645
  39. Farstad W. Current state in biotechnology in canine and feline reproduction. Anim Reprod Sci. 2000;60–61:375–387
  40. Goodrowe KL, Hay M. Characteristics and zona binding ability of fresh and cooled domestic cat epididymal spermatozoa. Theriogenology. 1993;40:967–975

PII: S0093-691X(10)00053-1

doi: 10.1016/j.theriogenology.2009.12.016

Theriogenology
Volume 74, Issue 1 , Pages 31-39 , 1 July 2010

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