Theriogenology
Volume 73, Issue 9 , Pages 1293-1305 , June 2010

Extremely low frequency electromagnetic field exposure affects fertilization outcome in swine animal model

Received 19 October 2009 ,Revised 23 December 2009 ,Accepted 27 December 2009.

References 

  1. European Commission. CORDIS. Seventh Framework Programme (FP7). http://www.cordis.europa.eu/fp5,6,7. Accessed: 9 September 2009.
  2. Wertheimer N, Leeper E. Electrical wiring configurations and childhood cancer. Am J Epidemiol. 1979;109:273–284
  3. Loscher W, Mevissen M. Animal studies on the role of 50/60- hertz magnetic fields in carcinogenesis. Life Sci. 1994;54:1531–1533
  4. Hlmberg B. Magnetic field and cancer: animal and cellular evidence—an overview. Environ Health Perspect. 1995;103:63–67
  5. Report NIESH. Health effect from exposure to power line frequency electric and magnetic fields. NIESH Publication. 1999;99:4493
  6. IARC. Monographs on the evaluation of carcinogenic risks to humans: non-ionizing radiation. Part. 1. Static and extremely low frequency (ELF) electric and magnetic fields, 2002.
  7. Huuskonen H, Juutilainen J, Komulainen H. Effects of low-frequency magnetic fields on fetal development in rats. Bioelectromagnetics. 1993;14:205–213
  8. Bracken MB, Belanger K, Hellenbrand K, Dlugosz L, Holfprd TR. Exposure to electromagnetic fields during pregnancy with emphasis on electrically heated beds: association with birth weight and intrauterine growth retardation. Epidemiology. 1995;6:263–270
  9. Li DK, Odouli R, Wi S, Janevic T, Golditch I. A population-based prospective cohort study of personal exposure to magnetic fields during pregnancy and the risk of miscarriage. Epidemiology. 2002;13:9–20
  10. Harmanci H, Emre M, Gurvit H, Bilgic B, Hanagasi H, Gurol , et al. Risk factors for Alzheimer disease: a population-based case-control study in Istanbul, Turkey. Alzheimer Dis Assoc Disord. 2003;17:139–145
  11. Håkansson N, Gustavsson P, Johansen C, Floderus B. Neurodegenerative diseases in welders and other workers exposed to high levels of magnetic fields. Epidemiology. 2003;14:420–428
  12. Feychting M, Jonsson F, Pedersen NL, Ahlbom A. Occupational magnetic field exposure and neurodegenerative disease. Epidemiology. 2003;14:413–419
  13. Sikov MR, Rommereim DN, Beamer JL, Buschbom RL, Kaune WT, Phillips RD. Developmental studies of Hanford miniature swine exposed to 60-Hz electric fields. Bioelectromagnetics. 1987;8:229–242
  14. Rommereim DN, Rommereim RL, Sikov MR, Buschbom RL, Anderson LE. Reproduction, growth, and development of rats during chronic exposure to multiple field strengths of 60-Hz electric fields. Fundam Appl Toxicol. 1990;14:608–621
  15. Mevissen M, Buntenkötter S, Löscher W. Effects of static and time-varying (50-Hz) magnetic fields on reproduction and foetal development in rats. Teratology. 1994;50:229–237
  16. Beraldi R, Sciamanna I, Mangiacasale R, Lorenzini R, Spadafora C. Mouse early embryos obtained by natural breeding or in vitro fertilization display a differential sensitivity to extremely low-frequency electromagnetic fields. Mutat Res. 2003;538:163–170
  17. Ivanova LA, Kartashev AG. The effect of alternating electric field of industrial frequency on testicles of white mice. Fiziol Zh. 1991;37:71–78
  18. De Vita R, Cavallo D, Raganella L, Eleuteri P, Grollino MG, Calugi A. Effects of 50Hz magnetic fields on mouse spermatogenesis monitored by flow cytometric analysis. Bioelectromagnetics. 1995;16:330–334
  19. Al-Akhras MA, Elbetieha A, Hasan MK, Al-Omari I, Darmani H, Albiss B. Effects of extremely low frequency magnetic field on fertility of adult male and female rats. Bioelectromagnetics. 2001;22:340–344
  20. Milunsky A, Ulcickas M, Rithman KJ, Willet W, Jick SS, Jick H. Maternal heat exposure and neural tube defects. Teratology. 1992;46:341–434
  21. Shaw GM, Nelson V, Todoroff K, Wasserman CR, Neutra RR. Maternal periconceptional use of electric bed-heating devices and risk for neural tube defects and orofacial clefts. Teratology. 1999;60:124–129
  22. WHO Environmental Health Criteria 238 EXTREMELY LOW FREQUENCY FIELDS. 2007.
  23. Blackman CF, Blanchard JP, Benane SG, House DE, Elder JA. Double blind test of magnetic field effects on neurite outgrowth. Bioelectromagnetics. 1998;19:204–209
  24. Khalil AM, Qassem W. Cytogenetic effects of pulsing electromagnetic field on human lymphocytes in vitro: chromosome aberrations, sister-chromatid exchanges and cell kinetics. Mutat Res. 1991;247:141–146
  25. Paradisi S, Donelli G, Santini MT, Straface E, Malori WA. 50-Hz magnetic field induces structural and biophysical changes in membranes. Bioelectromagnetics. 1993;14:247–255
  26. Goodman R, Wei LX, Xu JC, Anderson SC. Exposure of human cells to low-frequency electromagnetic field results in quantitative changes in transcripts. Biochim Biophys Acta. 1989;1009:216–220
  27. Karabakhtsian R, Broude N, Shalts N, Kochlatyi S, Goodman R, Henderson AS. Calcium is necessary in the cell response to EM fields. FEBS Lett. 1994;349:1–6
  28. Ivancsits S, Diem E, Pilger A, Rudiger HW, Jahn O. Induction of DNA strand breaks by intermittent exposure to extremelylow-frequency electromagnetic fields in human fibroblasts. Mutat Res. 2002;26:1–13
  29. Wolf FI, Torsello A, Tedesco B, Fasanella B, Boninsegna A, D’Ascenzo , et al. 50-Hz extremely low frequency electromagnetic field enhance cell proliferation and DNA damage: possible involvement of a redox mechanism. Biochim Biophys Acta. 2005;1743:120–129
  30. Winker R, Ivancsits S, Pilger A, Adlkofer F, Rüdiger HW. Chromosomal damage in human diploid fibroblasts by intermittent exposure to extremely low-frequency electromagnetic fields. Mutat Res. 2005;585:43–49
  31. Yokus B, Akdag MZ, Dasdag S, Cakir DU, Kizil M. Extremely low frequency magnetic fields cause oxidative DNA damage in rats. Int J Radiat Biol. 2008;84:789–795
  32. Hisamitsu T, Marita K, Kasahara T, Seto A, Yu T, Asano K. Induction of apoptosis in human leukemic cells by magnetic fields. Jpn J Physiol. 1997;47:307–310
  33. Adey WR, Sheppard A. In:  Blank M,  Find LE editor. Mechanistic approaches to interactions of electromagnetic fields with living systems. New York, USA: Plenum Press; 1987;p. 365–387
  34. Scaiano JC, Cozens FL, Mohtat N. Influence of combined ac–dc magnetic fields on free radicals in organized and biological systems. Development of a model and application of the radical pair mechanism to radicals in micelles. Photochem Photobiol. 1995;2:818–829
  35. Simkò M, Droste S, Kriehuber R, Weiss DG. Stimulation of phagocytosis and free radical production in murine macrophages by 50Hz electromagnetic fields. Eur J Cell Biol. 2001;80:562–566
  36. Rollwitz J, Lupke M, Simko‘ M. Fifty-hertz fields induce radical formation in mouse bone marrow-derived promyocytes and macrophages. Biochim Biophys Acta. 2004;1674:231–238
  37. Bernabo‘ N, Tettamanti E, Pistilli MG, Nardinocchi D, Berardinelli P, Mattioli M, et al. Effects of 50Hz extremely low frequency magnetic field on the morphology and function of boar spermatozoa capacitated in vitro. Theriogenology. 2007;67:801–815
  38. Maccarrone M, Barboni B, Paradisi A, Bernabò N, Gasperi V, Pistilli , et al. Characterization of the endocannabinoid system in boar spermatozoa and implications for sperm capacitation and acrosome reaction. J Cell Sci. 2005;118:4393–4404
  39. Kao SH, Chao HT, Wei YH. Multiple deletions of mitochondrial DNA are associated with the decline of motility and fertility of human spermatozoa. Mol Hum Reprod. 1998;4:657–666
  40. Mattioli M, Lucidi P, Barboni B. Expanded cumuli induce acrosome reaction in boar sperm. Mol Reprod Dev. 1998;51:445–453
  41. Barboni B, Mattioli M, Seren E. Influence of progesterone on boar sperm capacitation. J Endocrinol. 1995;144:1–8
  42. Castellani-Ceresa L, Mattioli M, Radaelli G, Barboni B, Brivio MF. Actin polymerization in boar spermatozoa: fertilization is reduced with use of cytochalasin D. Mol Reprod Dev. 1993;36:203–211
  43. Abeydeera LR, Day BN. In vitro penetration of pig oocytes in a modified Tris-buffered medium: effect of BSA, caffeine and calcium. Theriogenology. 1997;48:537–544
  44. Abeydeera LR, nad Day BN. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol Reprod. 1997;57:729–734
  45. Barboni B, Martelli A, Berardinelli P, Russo V, Turriani M, Bernabò N, et al. Ovarian follicle vascularization in fasted pig. Theriogenology. 2004;62:943–957
  46. Martelli A, Berardinell P, Russo V, Mauro A, Bernabò N, Gioia L, et al. Spatio-temporal analysis of vascular endothelial growth factor expression and blood vessel remodelling in pig ovarian follicles during the periovulatory period. J Mol Endocrinol. 2006;36:107–119
  47. Mateusen B, Van Soom A, Maes D, Donnay I, Duchateau L, Lequarre A. Porcine embryo development and fragmentation and their relation to apoptotic markers: a cinematographic and confocal laser scanning microscopic study. Reproduction. 2005;129:443–452
  48. Panagopoulos DJ, Messini N, Karabarbounis A, Philippetis AL, Margaritis LH. A mechanism for action of oscillating electric fields on cells. Bioch Bioph Research Comm. 2000;272:634–640
  49. Panagopoulos DJ, Karabarbounis A, Margaritis LH. Mechanism for action of electromagnetic fields on cells. Biochem Biophys Res Commun. 2002;298:95–102
  50. De Gennes PG. The physics of liquid crystals. Oxford: Clarendon Press; 1974;
  51. Blinov LM. Electro-optical and magneto-optical principles of liquid crystals. London: John Wiley and Sons; 1983;
  52. Ramundo-Orlando A, Morbiducci U, Mossa G, D’Inzeo G. Effect of low frequency, low amplitude magnetic fields on the permeability of cationic liposomes entrapping carbonic anhydrase.I. Evidence for charged lipid involvement. Bioelectromagnetics. 2000;21:491–498
  53. Fléchon JE, Hunter RH. Distribution of spermatozoa in the utero-tubal junction and isthmus of pigs, and their relationship with the luminal epithelium after mating: a scanning electron microscope study. Tissue Cell. 1981;13:127–139
  54. Hunter RH. The fallopian tubes: Their role in fertility and infertility. Berlin: Springer-Verlag; 1988;
  55. Hunter RH, Rodriguez-Martinez H. Capacitation of mammalian spermatozoa in vivo, with a specific focus on events in the Fallopian tubes. Mol Reprod Dev. 2004;67:243–250
  56. Hunter RH. The Fallopian tubes in domestic mammals: how vital is their physiological activity?. Reprod Nutr Dev. 2005;45:281–290
  57. Kouba A, Abeydeera A, Alvarez I, Day A, Buhi W. Effects of the Porcine Oviduct-Specific Glycoprotein on Fertilization, Polyspermy, and Embryonic Development In Vitro. Biol Reprod. 2000;63:242–250
  58. McCauley T, Buhi M, Wu G, Mao J, Caaman J, Didion B, et al. Oviduct-Specific Glycoprotein Modulates Sperm-Zona Binding and Improves efficiency of Porcine Fertilization In Vitro. Biol Reprod. 2003;69:828–834
  59. Coy P, Cánovas S, Mondéjar I, Saavedra MD, Romar R, Grullón L, et al. Oviduct-specific glycoprotein and heparin modulate sperm-zona pellucida interaction during fertilization and contribute to the control of polyspermy. Proc Natl Acad Sci USA. 2008;105:15809–15814
  60. Hardy K, Spanos S. Growth factors and function in the human and mouse preimplantation embryo. J Endocrionl. 2002;172:221–236
  61. Cleary SF. A review of in vitro studies: low-frequency electromagnetic fields. Am Ind Hyg Assoc J. 1993;54:178–185
  62. Saffer JD, Phillips JL. Evaluating the biological aspects of in vitro studies in bioelectromagnetics. Bioelectrochem Bioenerget. 1996;40:1–7
  63. Weaver CW. Understanding conditions for which biological effects of nonionizing electromagnetic fields can be expected. Bioelectrochem. 2002;56:207–209
  64. ICNIRP Guidelines on limits of exposure to static magnetic fields, 2008.
  65. WHO Fact Sheet N205. Available at: http://www.who.int/docstore/peh-MF/publications/facts_press/efact/efs205.html. Accessed 9 September 2009.
  66. NIEHS. Available at: http://www.niehs.nih.gov/MFrapid/html/Q&AWorkplace.html. Accessed 9 September 2009.

PII: S0093-691X(10)00047-6

doi: 10.1016/j.theriogenology.2009.12.010

Theriogenology
Volume 73, Issue 9 , Pages 1293-1305 , June 2010

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