Theriogenology
Volume 73, Issue 8 , Pages 1076-1087 , May 2010

Cryopreservation of epididymal cat spermatozoa: effects of in vitro antioxidative enzymes supplementation and lipid peroxidation induction

  • P. Thuwanut

      Affiliations

    • Division of Reproduction, Department of Clinical Sciences, P.O. Box 7054, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden
    • Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
    • ,
  • K. Chatdarong

      Affiliations

    • Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
    • ,
  • A. Johannisson

      Affiliations

    • Department of Physiology, Anatomy and Biochemistry, P.O. Box 7011, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden
    • ,
  • A.-S. Bergqvist

      Affiliations

    • Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
    • ,
  • L. Söderquist

      Affiliations

    • Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
    • ,
  • E. Axnér

      Affiliations

    • Division of Reproduction, Department of Clinical Sciences, P.O. Box 7054, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden
    • Corresponding Author InformationCorresponding author. Tel.: +46 18 67 21 81; fax: +46 18 67 35 45.

Received 17 June 2009 ,Revised 21 December 2009 ,Accepted 9 January 2010.

References 

  1. Luvoni GC, Kalchschmidt E, Marinoni G. Conservation of feline semen. Part II: Cold-induced damages on spermatozoal fertilizing ability. J Feline Med Surg. 2003;5:257–263
  2. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril. 2003;79:829–843
  3. Silva PFN. Physiology of peroxidation process in mammalian sperm. PhD thesis. Utrech University, Ridderprint, Ridderkerk, 2006, pp. 5–36.
  4. Aitken RJ. Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev. 1995;7:659–668
  5. Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA. Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Mol Hum Reprod. 2007;13:203–211
  6. Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med. 1995;18:321–336
  7. Sikka SC. Relative impact of oxidative stress on male reproductive function. Curr Med Chem. 2001;8:851–862
  8. Jelezarsky L, Vaisberg Ch, Chaushev T, Sapundjev E. Localization and characterization of glutathione peroxidase (GPx) in boar accessory glands, seminal plasma, and spermatozoa and activity of GPx in boar semen. Theriogenology. 2008;69:139–145
  9. Michael AJ, Alexopoulos C, Pontiki EA, Hadjipavlou-Litina DJ, Saratsis P, Ververidis HN, et al. Quality and reactive oxygen species of extended canine semen after vitamin C supplementation. Theriogenology. 2008;70:827–835
  10. Thuwanut P, Chatdarong K, Techakumphu M, Axnér E. The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa. Theriogenology. 2008;70:233–240
  11. Funahashi H, Sano T. Select antioxidants improve the function of extended boar semen stored at 10°C. Theriogenology. 2005;63:1605–1616
  12. Luvoni GC, Ruggiero G, Marinoni G, Kalchschmidt E. Effect of taurine containing diluent for cryopreservation of domestic cat epididymal sperm. Theriogenology. 2002;57:466
  13. Maxwell WM, Stojanov T. Liquid storage of ram semen in the absence or presence of some antioxidants. Reprod Fertil Dev. 1996;8:1013–1020
  14. Maria R, Fernández-Santos MR, Martínez-Pastor F, García-Macías V, Esteso MC, Soler AJ, et al. Sperm characteristics and DNA integrity of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa frozen in the presence of enzymatic and nonenzymatic antioxidants. J Androl. 2007;28:294–305
  15. Michael A, Alexopoulos C, Pontiki E, Hadjipavlou-Litina D, Saratsis P, Boscos C. Effect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology. 2007;68:204–212
  16. Bolle P, Evandri MG, Saso L. The controversial efficacy of vitamin E for human male infertility. Contraception. 2002;65:313–315
  17. Axnér E, Hermansson U, Linde-Forsberg C. The effect of Equex STM paste and sperm morphology on frozen-thawed survival of cat epididymal sperm. Anim Reprod Sci. 2004;84:179–191
  18. Rota A, Strom B, Linde-Forsberg C. Rodiguez Martinez H. Effects of equex STM paste on viability of frozen-thawed dog sperm during in vitro incubation at 38°C. Theriogenology. 1997;47:1093–1101
  19. Lagerlöf N. Morphological studies on the change in sperm structure and in the testes of bulls with decreased or abolished fertility. Acta Pathol Microbiol Scand Suppl. 1934;19:254–267
  20. Bane A. Acrosomal abnormality associated with sterility in a boar. Proceedings of the 4th International Congress in Animal Reproduction and AI, The Hague, June, 1961. p. 810–17.
  21. Bussalleu E, Pinart E, Yeste M, Briz M, Sancho S, Garcia-Gil N, et al. Development of a protocol for multiple staining with fluorochromes to assess the functional status of boar spermatozoa. Microsc Res Tech. 2005;68:277–283
  22. Thuwanut P, Axnér E, Johanisson S, Chatdarong K. Detection of lipid peroxidation reaction in frozen-thawed epididymal cat spermatozoa using BODIPY581/591 C11. Reprod Domest Anim. 2009;44(Suppl 2):373–376
  23. Kankofer M, Kolm G, Aurich J, Aurich C. Activity of glutathione peroxidase, super oxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5°C. Theriogenology. 2005;63:1354–1365
  24. Nair SJ, Brar AS, Ahuja CS, Sangha SP, Chaudhary KC. A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature. Anim Reprod Sci. 2006;96:21–29
  25. Baumber J, Ball BA, Linfor JJ. Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants. Am J Vet Res. 2005;66:772–779
  26. Thiangtum K, Pinyopummintr A, Hori T, Kawakami E, Tsutsui S. Effect of catalase and superoxide dismutase on motility, viability and acrosomal integrity of frozen-thawed cat spermatozoa. Proceeding of 6th International symposium on canine and feline reproduction and 6th Biannual EVSSAR congress. Austria. 2008. p. 252–3.
  27. Mortimer ST. A critical review of the physiological importance and analysis of sperm movement in mammals. Hum Reprod Update. 1997;3:403–439
  28. Filliers M, Rijsselaere T, Bossaert P, De Causmaecker V, Dewulf J, Pope CE, et al. Computer-assisted sperm analysis of fresh epididymal cat spermatozoa and the impact of cool storage (4 degrees C) on sperm quality. Theriogenology. 2008;70:1550–1559
  29. Bilodeau JF, Blanchette S, Gagnon C, Sirard MA. Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology. 2001;56:275–286
  30. Drevet JR. The antioxidant glutathione peroxidase family and spermatozoa: A complex story. Mol Cell Endocrinol. 2006;250:70–79
  31. Agrawal YP, Vanha-Perttula T, Glutathione . L-glutamic acid and gramma-glutamyl transpeptidase in the bull reproductive tissues. Int J Androl. 1988;11:123–131
  32. Irvine DS. Glutathione as a treatment for male infertility. Rev Reprod. 1996;1:6–12
  33. Jeong BS, Yang X. Cysteine, glutathione, and Percoll treatments improve porcine oocyte maturation and fertilization in vitro. Mol Reprod Dev. 2001;59:330–335
  34. O’Flaherty CM, Beorlegui NB, Beconi MT. Reactive oxygen species requirements for bovine sperm capacitation and acrosome reaction. Theriogenology. 1999;52:289–301
  35. Hsu PC, Hsu CC, Guo YL. Hydrogen peroxide induces premature acrosome reaction in rat sperm and reduces their penetration of the zona pellucida. Toxicology. 1999;139:93–101
  36. Peris SI, Bilodeau JF, Dufour M, Bailey JL. Impact of cryopreservation and reactive oxygen species on DNA integrity, lipid peroxidation, and functional parameters in ram sperm. Mol Reprod Dev. 2007;74:878–892
  37. Rossi T, Mazzilli F, Delfino M, Dondero F. Improved human sperm recovery using superoxide dismutase and catalase supplementation in semen cryopreservation procedure. Cell Tissue Bank. 2001;2:9–13

PII: S0093-691X(10)00027-0

doi: 10.1016/j.theriogenology.2010.01.007

Theriogenology
Volume 73, Issue 8 , Pages 1076-1087 , May 2010

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