Theriogenology
Volume 73, Issue 9 , Pages 1180-1191 , June 2010

In vitro induction of the acrosome reaction in bull sperm and the relationship to field fertility using low-dose inseminations

  • A. Birck

      Affiliations

    • Copenhagen University, Faculty of Life Sciences, Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Frederiksberg, Denmark
    • Present address: SPZ Lab A/S, Fruebjergvej 3, 2100 Copenhagen OE, Denmark.
    • Corresponding Author InformationCorresponding author. Tel.: +45 39 17 97 84; fax: +45 39 17 97 66.
    • ,
  • P. Christensen

      Affiliations

    • Copenhagen University, Faculty of Life Sciences, Department of Large Animal Sciences, Veterinary Reproduction and Obstetrics, Frederiksberg, Denmark
    • Present address: SPZ Lab A/S, Fruebjergvej 3, 2100 Copenhagen OE, Denmark.
    • ,
  • R. Labouriau

      Affiliations

    • Aarhus University, Faculty of Agricultural Sciences, Department of Genetics and Biotechnology, Tjele, Denmark
    • ,
  • J. Pedersen

      Affiliations

    • The Danish Cattle Federation, Danish Agricultural Advisory Service, Aarhus, Denmark
    • ,
  • S. Borchersen

      Affiliations

    • Dansire, Randers, Denmark

Received 1 February 2009 ,Revised 20 September 2009 ,Accepted 11 October 2009.

References 

  1. Correa JR, Zavos PM. The hypoosmotic swelling test: its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenology. 1994;42:351–360
  2. Fazeli A, Zhang BR, Steenweg W, Larsson B, Bevers MM, van den BJ, et al. Relationship between sperm-zona pellucida binding assays and the 56-day nonreturn rate of cattle inseminated with frozen-thawed bull semen. Theriogenology. 1997;48:853–863
  3. Holt C, Holt WV, Moore HD, Reed HC, Curnock RM. Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. J Androl. 1997;18:312–323
  4. Murase T, Okuda K, Sato K. Assessment of bull fertility using a mucus penetration test and a human chorionic gonadotrophin stimulation test. Theriogenology. 1990;34:801–812
  5. Pace MM, Graham EF. The release of glutamic oxaloacetic transaminase from bovine spermatozoa as a test method of assessing semen quality and fertility. Biol Reprod. 1970;3:140–146
  6. Whitfield CH, Parkinson TJ. Relationship between fertility of bovine semen and in vitro induction of acrosome reactions by heparin. Theriogenology. 1992;38:11–20
  7. Barth AD. Abnormal Morphology of Bovine Spermatozoa. Iowa State University Press; 1989;
  8. Blom E. A one-minute live-dead stain by means of eosin-nigrosin. Fertil Steril. 1950;1:176–177
  9. Budworth PR, Amann RP, Chapman PL. Relationships between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility. J Androl. 1988;9:41–54
  10. Graham JK. Assessment of sperm quality: a flow cytometric approach. Anim Reprod Sci. 2001;68:239–247
  11. Evenson DP, Darzynkiewicz Z, Melamed MR. Simultaneous measurement by flow-cytometry of sperm cell viability and mitochondrial-membrane potential related to cell motility. J Histochem Cytochem. 1982;30:279–280
  12. Evenson DP. Flow cytometric analysis of chromatin damage in sperm isolated from mice exposed to toxic-chemicals. Environ Mutagen. 1984;6:404–405
  13. Garner DL, Johnson LA. Viability assessment of mammalian sperm using SYBR-14 and prodidium iodide. Biol Reprod. 1995;53:276–284
  14. Garner DL, Thomas CA, Joerg HW, DeJarnette JM, Marshall CE. Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol Reprod. 1997;57:1401–1406
  15. Nagy S, Jansen J, Topper EK, Gadella BM. A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod. 2003;68:1828–1835
  16. Yanagimachi R. Mammalian fertilization. In:  Knobil E,  Neill JD editor. The Physiology of Reproduction. Raven Press Ltd; 1994;p. 189–317
  17. Babcock DF, First NL, Lardy HA. Action of ionophore A23187 at cellular level - separation of effects at plasma and mitochondrial-membranes. J Biol Chem. 1976;251:3881–3886
  18. Dedkova EN, Sigova AA, Zinchenko VP. Mechanism of calcium ionophores on the intact cells. Ionophore-resistant cells. Biol Membrany. 1999;16:292–301
  19. Talbot P, Summers RG, Hylander BL, Keough EM, Franklin LE. Role of calcium in acrosome reaction - analysis using ionophore A23187. J Exp Zool. 1976;198:383–392
  20. Flesch FM, Gadella BM. Dynamics of the mammalian sperm plasma membrane in the process of fertilization. Biochim Biophys Acta Rev Biomembranes. 2000;1469:197–235
  21. Bastiaan HS, Windt ML, Menkveld R, Kruger TF, Oehninger S, Franken DR. Relationship between zona pellucida-induced acrosome reaction, sperm morphology, sperm-zona pellucida binding, and in vitro fertilization. Fertil Steril. 2003;79:49–55
  22. Franken DR, Bastiaan HS, Kidson A, Wranz P, Habenicht UF. Zona pellucida mediated acrosome reaction and sperm morphology. Andrologia. 1997;29:311–317
  23. Januskauskas A, Johannisson A, Soderquist L, Rodriguez-Martinez H. Assessment of sperm characteristics post-thaw and response to calcium ionophore in relation to fertility in Swedish dairy AI bulls. Theriogenology. 2000;53:859–875
  24. Meyers SA, Overstreet JW, Liu IK, Drobnis EZ. Capacitation in vitro of stallion spermatozoa: comparison of progesterone-induced acrosome reactions in fertile and subfertile males. J Androl. 1995;16:47–54
  25. Meyers SA, Liu IKM, Overstreet JW, Vadas S, Drobnis EZ. Zona pellucida binding and zona-induced acrosome reactions in horse spermatozoa: comparisons between fertile and subfertile stallions. Theriogenology. 1996;46:1277–1288
  26. Whitfield CH, Parkinson TJ. Assessment of the fertilizing potential of frozen bovine spermatozoa by in-vitro induction of acrosome reactions with calcium ionophore (A23187). Theriogenology. 1995;44:413–422
  27. Birck A. The Acrosome Reaction—Its Induction, Detection and Relationship to Fertility. Dissertation, Veterinary Reproduction and Obstetrics, Faculty of Life Science, University of Copenhagen, 2007.
  28. Amann RP. Can the fertility potential of a seminal sample be predicted accurately. J Androl. 1989;10:89–98
  29. den Daas JH, De JG, Lansbergen LM, van Wagtendonk-de Leeuw AM. The Physiology of Reproduction The relationship between the number of spermatozoa inseminated and the reproductive efficiency of individual dairy bulls. J Dairy Sci. 1998;81:1714–1723
  30. Christensen P, Stenvang JP, Godfrey WL. A flow cytometric method for rapid determination of sperm concentration and viability in mammalian and avian semen. J Androl. 2004;25:255–264
  31. Labouriau R, Christensen P, Borchersen S. Considerations on the sample design of an experiment on semen dilution for artificial insemination in cattle. Internal report, Biometry Research Unit, Danish Institute of Agricultural Sciences, 2003. Available at: http://gbi.agrsci.dk/∼rsl/Publications/2003f/2003f.pdf.
  32. Tyrode MV. The mode of action of some purgative salts. Arch Intern Pharmacodyn. 1910;17:205–209
  33. Thomas CA, Garner DL, DeJarnette JM, Marshall CE. Fluorometric assessments of acrosomal integrity and viability in cryopreserved bovine spermatozoa. Biol Reprod. 1997;56:991–998
  34. Davidson AC, Hinkley DV. The Physiology of Reproduction Bootstrap Methods and Their Application. Cambridge University Press; 1997;
  35. Hastie T. Generalized additive models. In:  Chambers JM,  Hastie T editor. Statistical Models. Wadsworth & Brooks; 1990;p. 249–308Chapter 7
  36. Hastie T, Tibshirani R. Generalized Additive Models. Chapman and Hall; 1990;
  37. Labouriau R. Estimating Functions and Semiparametric Models. Dissertation, Department of Theoretical Statistics, Aarhus University, 1998.
  38. Aitken RJ, Buckingham DW, Fang HG. Analysis of the responses of human spermatozoa to A23187 employing a novel technique for assessing the acrosome reaction. J Androl. 1993;14:132–141
  39. Auger J, Eustache F, Ducot B, Blandin T, Daudin M, Diaz I, et al. Intra- and inter-individual variability in human sperm concentration, motility and vitality assessment during a workshop involving ten laboratories. Hum Reprod. 2000;15:2360–2368
  40. Graham JK, Kunze E, Hammerstedt RH. Analysis of sperm cell viability, acrosomal integrity, and mitochondrial-function using flow-cytometry. Biol Reprod. 1990;43:55–64
  41. Garner DL, Pinkel D, Johnson LA, Pace MM. Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analyses. Biol Reprod. 1986;34:127–138
  42. Byrd W, Tsu J, Wolf DP. Kinetics of spontaneous and induced acrosomal loss in human-sperm incubated under capacitating and noncapacitating conditions. Gamete Res. 1989;22:109–122
  43. De Jonge CJ. The diagnostic significance of the induced acrosome reaction. Reprod Med Rev. 1994;3:159–178
  44. Esterhuizen AD, Franken DR, Lourens JGH, Van Rooyen LH. Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF. Hum Reprod. 2001;16:138–144
  45. Cummins JM, Pember SM, Jequier AM, Yovich JL, Hartmann PE. A test of the human sperm acrosome reaction following ionophore challenge - relationship to fertility and other seminal parameters. J Androl. 1991;12:98–103
  46. Matson PL. Clinical value of tests for assessing male infertility. Baillieres Clin Obstet Gynaecol. 1997;11:641–654
  47. Petrunkina AM, Volker G, Brandt H, Topfer-Petersen E, Waberski D. Functional significance of responsiveness to capacitating conditions in boar spermatozoa. Theriogenology. 2005;64:1766–1782
  48. Blondin P, Beaulieu M, Fournier V, Morin N, Crawford L, Madan P, et al. Analysis of bovine sexed sperm for IVF from sorting to the embryo. Theriogenology. 2009;71:30–38
  49. Lu KH, Seidel GE. Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm. Theriogenology. 2004;62:819–830

PII: S0093-691X(09)00485-3

doi: 10.1016/j.theriogenology.2009.10.010

Theriogenology
Volume 73, Issue 9 , Pages 1180-1191 , June 2010

Article Tools

* Image Usage & Resolution