Theriogenology
Volume 73, Issue 3 , Pages 316-323 , February 2010

Effects of different extenders and centrifugation/washing on postthaw microscopic-oxidative stress parameters and fertilizing ability of Angora buck sperm

  • S. Sarıözkan

      Affiliations

    • Erciyes University, Safiye Cikrikcioglu Vocational College, Kayseri, Turkey
    • Corresponding Author InformationCorresponding author. Tel.: +90 352 437 52 77; fax: +90 352 437 13 83.
  • ,
  • M.N. Bucak

      Affiliations

    • Ministry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Lalahan, Ankara, Turkey
  • ,
  • P.B. Tuncer

      Affiliations

    • Ministry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Lalahan, Ankara, Turkey
  • ,
  • U. Taşdemir

      Affiliations

    • Ministry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Lalahan, Ankara, Turkey
  • ,
  • H. Kinet

      Affiliations

    • Ministry of Agriculture and Rural Affairs, Lalahan Livestock Central Research Institute, Lalahan, Ankara, Turkey
  • ,
  • P.A. Ulutaş

      Affiliations

    • Department of Biochemistry, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey

Received 7 July 2009 ,Revised 4 September 2009 ,Accepted 8 September 2009.

References 

  1. Evans G, Maxwell WMC. Introduction to artificial insemination. In: Salamon's Artificial Insemination of Sheep and Goats, Maxwell WMC (Ed.), Butterworths, 1987, pp. 1–7.
  2. Vishwanath R, Shannon P. Storage of bovine semen in liquid and frozen state. Anim Reprod Sci. 2000;62:23–53
  3. Ritar AJ, Ball PD, O’May PJ. Artificial insemination of cashmere goats: effects on fertility and fecundity of intravaginal treatment, method and time of insemination, semen freezing process,;1; number of motile spermatozoa and age of females. Reprod Fertil Dev. 1990;2:377–384
  4. Ritar AJ, Salamon S. Fertility of fresh and frozen-thawed semen of Angora goat. Aust J Biol Sci. 1983;36:49–59
  5. Ghalsasi PM, Nimbkar C. Evaluation of laparoscopic intraurine insemination in ewes. Small Rumin Res. 1996;23:69–73
  6. Moore RW, Miller CM, Hall DR. Cervical versus laparoscopic AI of goat after PMSG injection at or 48hours before CDIR removal. Proc New Zealand Soc Anim Production. 1988;48:69–70
  7. Leboeuf B, Restall B, Salamon S. Production and storage of goat semen for artificial insemination. Anim Reprod Sci. 2000;62:113–141
  8. Upreti GC, Hall EL, Koppens D, Oliver JE, Vishwanath R. Studies on the measurement of phospholipase A2 (PL A2) and PL A2 inhibitor activities in ram semen. Anim Reprod Sci. 1999;56:107–121
  9. Iritani A, Nishikawa Y. Studies on the egg-coagulating enzyme in goat semen. IV. On the position of yolk constituents attacked by the coagulating enzyme. Jap J Anim Reprod. 1963;8:13–117
  10. Amoah EA, Gelaye S. Biotechnological advances in goat reproduction. J Anim Sci. 1997;75:578–585
  11. Ritar AJ, Salamon S. Effects of seminal plasma and of its removal and egg yolk in the diluent on the survival of fresh and frozen-thawed spermatozoa of the Angora goat. Aust J Biol Sci. 1982;35:305–312
  12. Memon MA, Bretzlaff KN, Ott RS. Effect of washing on motility and acrosome morphology of frozen-thawed goat spermatozoa. Am J Vet Res. 1985;46:473–475
  13. Kozdrowski R, Dubiel A, Bielas W, Dzieciol M. Two protocols of cryopreservation of goat semen with the use of computer-assisted semen analyses system. Acta Vet Brno. 2007;76:601–604
  14. Azeredo GA, Esper CR, Resende KT. Evaluation of plasma membrane integrity of frozen-thawed goat spermatozoa with or without seminal plasma. Small Rumin Res. 2001;41:257–263
  15. Bucak MN, Ateşşahin A, Varışlı Ö, Yüce A, Tekin N, Akçay A. The influence of trehalose, taurine, cysteamine and hyaluronan on ram semen: microscopic and oxidative stress parameters after the freeze-thawing process. Theriogenology. 2007;67:1060–1067
  16. Chatterjee S, De Lamirande E, Gagnon C. Cryopreservation alters membrane sulfydryl status of bull spermatozoa: protection by oxidized glutathione. Mol Reprod Dev. 2001;60:498–506
  17. Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennigs Z, et al. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod. 1998;59:1037–1046
  18. White IG. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev. 1993;5:639–658
  19. Zhao Y, Buhr MM. Cryopreservation extenders affect calcium flux in bovine spermatozoa during a temperature challenge. J Androl. 1995;16:278–285
  20. Aitken RJ, Fischer H. Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays. 1994;16:259–267
  21. Cassani P, Beconi MT, O’Flaherty C. Relationship between total superoxide dismutase activity with lipid peroxidation, dynamics and morphological parameters in canine semen. Anim Reprod Sci. 2005;86:163–173
  22. Sinha MP, Sinha AK, Singh BK, Prasad RL. The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Anim Reprod Sci. 1996;41:237–243
  23. Bucak MN, Sarıözkan S, Tuncer PB, Ulutaş PA, Akçadağ HA. Effects of an antioxidants on microscopic semen parameters, lipid peroxidation and antioxidant activities in Angora goat semen following cryopreservation. Small Rumin Res. 2009;81:90–95
  24. Salamon S, Ritar AJ. Deep freezing of Angora goat semen: effects of diluent composition and method and rate of dilution on survival of spermatozoa. Aust J Biol Sci. 1982;35:295–303
  25. Smith JT, Mayer DT. Evaluation of sperm concentration by the hemocytometer method. Fertil Steril. 1955;6:271–275
  26. Schafer S, Holzmann A. The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci. 2000;59:201–211
  27. Revell SG, Mrode RA. An osmotic resistance test for bovine semen. Anim Reprod Sci. 1994;36:77–86
  28. Buckett WM, Luckas MJ, Aird IA, Farquharson RG, Kingsland CR, Lewis-Jones DI. The hypo-osmotic swelling test in recurrent miscarriage. Fertil Steril. 1997;68:506–509
  29. Dandekar SP, Nadkarni GD, Kulkarni VS, Punekar S. Lipid peroxidation and antioxidant enzymes in male infertility. F Postgrad Med. 2002;48:186–189
  30. Kasimanicham R, Kasimanicham V, Thatcher CD, Nebel RL, Cassell BG. Relationships among lipid peroxidation, glutathione peroxidase, superoxide dismutase, sperm parameters, and competitive index in dairy bulls. Theriogenology. 2007;67:1004–1012
  31. Yoshioka T, Kawada K, Shimada S. Lipid peroxidation in maternal and cord blood and protective mechanism against activated oxygene toxicity in the blood. Am J Obstet Gynecol. 1979;135:372–376
  32. Sun M, Zigman S. Determination of superoxide dismutase in erythrocytes using the method of adrenaline autooxidation. Anal Biochem. 1978;90:81–89
  33. Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev. 2000;55:282–288
  34. Bilodeau JF, Blanchette S, Gagnon IC, Sirard MA. Thiols prevent H2O2-mediated loss of sperm motility in cryopreserved bull semen. Theriogenology. 2001;56:275–286
  35. Sarıözkan S, Bucak MN, Tuncer PB, Ulutaş PA, Bilgen A. The influence of cysteine and taurine on microscopic-oxidative stress parameters and fertilizing ability of bull semen following cryopreservation. Cryobiology. 2009;58:134–138
  36. Upreti GC, Jensen K, Munday R, Duganzich DM, Vishwanath R, Smith JF. Studies on aromatic amino acid oxidase activity in ram spermatozoa: role of pyruvate as an antioxidant. Anim Reprod Sci. 1998;51:275–287
  37. Gadea J, Sellens E, Marco MA, Coy P, Matas C, Romar R, et al. Decrease in glutathione content in boar sperm after cryopreservation. Effect of the addition of reduced glutathione to the freezing and thawing extenders. Theriogenology. 2004;62:690–701
  38. Funahashi H, Sano T. Selected antioxidants improve the function of extended boar semen stored at 10°C. Theriogenology. 2005;63:1605–1616
  39. Alvarez JG, Storey BT. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res. 1989;23:77–90
  40. Bell M, Wang R, Hellstrom WJ, Sikka SC. Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm. J Androl. 1993;14:472–478
  41. Chen H, Chow PH, Cheng SK, Cheung AL, Cheng LY, O WS. male genital tract antioxidant enzymes: their source, function in the female, and ability to preserve sperm DNA integrity in the golden hamster. J Androl 2003; 24:704–711.
  42. Verma A, Kanwar KC. Effect of vitamin E on human sperm motility and lipid peroxidation in vitro. Asian J Androl. 1999;1:151–154
  43. O WS, Chen C, Chow PH. Male genital tract antioxidant enzymes—their ability to preserve sperm DNA integrity. Mol Cell Endocrinol 2006; 250:80–83.
  44. Fernandez-Santoz MR, Martinez-Pastor F, Garcia-Macias V, Esteso MC, Soler AJ, Paz P, et al. Sperm characteristics and DNA integrity of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa frozen in the presence of enzymatic and non enzymatic antioxidants. J Androl. 2007;28:294–305
  45. Aurich JE, Schönherr U, Hoppe H, Aurich C. Effect of antioxidants on motility and membrane integrity of chilled-stored stallion semen. Theriogenology. 1997;48:185–192
  46. Fridovich I. Superoxide dismutase, regularities and irregularities. Harvey Lect. 1985;79:51–75
  47. Baker HWG, Brindle J, Irvine DS, Aitken J. Protective effect of antioxidants on the impairment of sperm motility by activated polymorphonuclear leukocytes. Fertil Steril. 1996;65:411–419
  48. Stradaioli G, Noro T, Sylla L, Monaci M. Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders. Theriogenology. 2007;67:1249–1255
  49. Daşkın A, Tekin N. The effect of egg yolk on the quality of frozen Angora goat semen. Tr J Vet Anim Sci. 1996;20:395–398
  50. Bucak MN, Tuncer PB, Sarıözkan S, Ulutaş PA, Çoyan K, Başpınar N, Özkalp B. Effects solution on hypotaurine, cysteamine and aminoacids post-thaw microscopic and oxidative stress parameters of Angora goat semen. Res Vet Sci 2009 (in press).
  51. Carnaval G, Cuello C, Ruiz M, Vazquez JM, Arsenıo M, Roca J. Effects of centrifugation before freezing on boar sperm cryosurvival. J Androl. 2004;25:389–396
  52. Gil J, Söderquist L, Rodriguez-Martinez H. Influence of centrifugation and different extenders on post-thaw sperm quality of ram semen. Theriogenology. 2000;54:93–108
  53. Gil J, Lundeheim N, Söderquist L, Rodriguez-Martinez H. Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in goat semen. Theriogenology. 2003;59:1241–1255
  54. Gil J, Rodriguez-Irazoqui M, Lundeheim N, Söderquist L, Rodriguez-Martinez H. Fertility of ram semen frozen in Bioxcell® and used for cervical artificial insemination. Theriogenology. 2003;59:1157–1170

PII: S0093-691X(09)00418-X

doi: 10.1016/j.theriogenology.2009.09.015

Theriogenology
Volume 73, Issue 3 , Pages 316-323 , February 2010