Theriogenology
Volume 73, Issue 3 , Pages 300-308 , February 2010

Vitrification and warming of in vivo–derived porcine embryos in a chemically defined medium

Received 25 June 2009 ,Revised 29 July 2009 ,Accepted 31 July 2009.

References 

  1. Vajta G, Nagy ZP. Are programmable freezers still needed in the embryo laboratory? Review on vitrification. Reprod Biomed Online. 2006;12:779–796
  2. Vajta G, Holm P, Greeve T, Callensen H. Vitrification of porcine embryos using the Open Pulled Straw (OPS) method. Acta Vet Scand. 1997;38:348–352
  3. Cuello C, Gil MA, Parrilla I, Tornel J, Vazquez JM, Roca J, et al. Vitrification of porcine embryos at various developmental stages using different ultra-rapid cooling procedures. Theriogenology. 2004;6:353–361
  4. Cuello C, Gil MA, Parrilla I, Tornel J, Vazquez JM, Roca J, et al. In vitro development following one-step dilution of OPS-vitrified porcine blastocysts. Theriogenology. 2004;62:1144–1152
  5. Berthelot F, Martinat-Botte F, Locatelli A, Perreau C, Terqui M. Piglets born after vitrification of embryos using the Open Pulled Straw method. Cryobiology. 2000;41:116–124
  6. Berthelot F, Martinat-Botte F, Perreau C, Terqui M. Birth of piglets after OPS vitrification and transfer of compacted morula stage with intact zona pellucida. Reprod Nutr Dev. 2001;41:267–272
  7. Berthelot F, Martinat-Botte F, Vajta G, Terqui M. Cryopreservation of porcine embryos: state of the art. Livest Prod Sci. 2003;83:73–83
  8. Beebe LF, Cameron RD, Blackshaw AW, Keates HL. Changes to porcine blastocyst vitrification methods and improved litter size after transfer. Theriogenology. 2005;64:879–890
  9. Cuello C, Berthelot F, Martinat-Botte F, Venturi E, Vazquez JM, Roca J, et al. Piglets born after non-surgical deep intrauterine transfer of vitrified blastocysts in gilts. Anim Reprod Sci. 2005;85:275–286
  10. Witkowski JA, Brighton WD. Influence of serum on attachment of tissue cells to glass. Exp Cell Res. 1972;70:41–48
  11. Leibo SP. Cryopreservation of embryos. In: XI Intern Cong Anim Reprod, Artificial Insemination, Vol 5, Dublin, Ireland, 1988, pp. 370–377.
  12. Shaw JM, Trouson AO. Effect of dimethylsulfoxide and protein concentration on the viability of two-cell mouse embryos frozen with rapid-freezing technique. Cryobiology. 1989;26:413–421
  13. Wowk B, Leitl E, Rasch CM, Mesbah-Karimi N, Harris SB, Fahy GM. Vitrification enhancement by sinthetic ice blocking agents. Cryobiology. 2000;40:228–236
  14. Kuleshova LL, Shaw JM, Trouson AO. Studies on replacing most of the penetrating cryoprotectant by polymers for embryo cryopreservation. Cryobiology. 2001;43:21–31
  15. Rossi CR, Bridgman BS, Kiesel GK. Viral contamination of bovine fetal lung cultures and bovine fetal serum. Am J Vet Res. 1980;41:1680–1681
  16. Thibier M, Nibart M. Disease control and embryo imports. Theriogenology. 1987;27:37–47
  17. Wrathall AE. Embryo transfer and disease transmission in livestock: a review of recent research. Theriogenology. 1995;43:81–88
  18. Guerin B, Nibart M, Marquant-Le Guienne B, Humblot P. Sanitary risks related to embryo transfer in domestic species. Theriogenology. 1997;47:33–42
  19. Creighton KA, Lindner GM. Effect of polyvinyl alcohol on in vitro survival of frozen-thawed mouse embryos [abstract]. Theriogenology. 1983;19:120
  20. Joly T, Nibart M, Thibier M. Hyaluronic acid as a substitute for proteins in the deep freezing of embryos from mice and sheep: an in vitro investigation. Theriogenology 1992;37:473–480.
  21. Gutierrez A, Garde J, Artiga CG, Muñoz I, Pintado B. In vitro survival of murinae morulae after quick freezing in the presence of chemically defined macromolecules and different cryoprotectants. Theriogenology. 1993;38:1111–1120
  22. Leibo SP, Odd K. High survival of mouse zygotes and embryos cooled rapidly or slowly in ethylene glycolplus polyvinyl pirrolidone. Cryo Letters. 1993;14:133–144
  23. Palasz A, Alkemade S, Mapletoft RJ. The use of sodiun hyaluronate in freezing media for bovine and murine embryos. Cryobiology. 1993;30:172–178
  24. Palasz AT, Thundathil J, De la Fuente J, Mapletoft RJ. Effect of reduced concentrations of glycerol and various macromolecules on the cryopreservation of mouse and cattle embryos. Cryobiology. 2000;41:35–42
  25. George F, Vrancken M, Verhaeghe B, Vehoeye F, Schneider YJ, Massip A, et al. Freezing of in vitro produced bovine embryos in animal protein-free medium containing vegetal peptones. Theriogenology. 2006;66:1381–1390
  26. Kim CG, Yong H, Lee G, Cho J. Effect of polyvinylpyrrolidone concentration of cryoprotectants on mouse embryo development and production of pups: 7.5% of PVP is beneficial for in vitro and in vivo development of frozen-thawed mouse embryos. J Reprod Dev. 2008;54:250–253
  27. Nowshari MA, Brem G. The protective action of polyvinyl alcohol during rapid-freezing of mouse embryos. Theriogenology. 2000;53:1157–1166
  28. Naitana S, Ledda S, Loi P, Leoni G, Bogliolo L, Dattena M, et al. Polyvinyl alcohol as defined substitute for serum in vitrification and warming solutions to cryopreserve ovine embryos at different stages of development. Anim Reprod Sci. 1997;48:247–256
  29. Vajta G, Rindom N, Peura TT, Holm P, Greeve T, Callesen H. The effect of media, serum and temperature on in vitro survival of bovine blastocyts after Open Pulled Straw (OPS) vitrification. Theriogenology. 1999;52:939–948
  30. Leoni G, Bogliolo L, Berlinguer F, Rosati I, Pintus PP, Ledda S, et al. Defined media for vitrification, warming and rehydration: effects on post-thaw protein synthesis and viability of in vitro derived ovine embryos. Cryobiology. 2002;45:204–212
  31. Pursel VG, Johnson LA. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and new training procedure. J Anim Sci. 1997;40:99–102
  32. Funahashi H, Ekwall H, Rodriguez-Martinez H. Zona reaction in porcine oocytes fertilized in vivo and in vitro as seen with scanning electron microscopy. Biol Reprod. 2000;63:1437–1442
  33. Merton S. Morphological evaluation of embryos in domestic species. In:  Van Soom A,  Boerjan M editor. Assessment of Mammalian Embryo Quality. Kluwer Academic Publishers; 2002;p. 33–35
  34. Sanchez-Osorio J, Cuello C, Gil MA, Almiñana C, Parrilla I, Caballero I, et al. Factors affecting the success rate of porcine embryo vitrification by the Open Pulled Straw method. Anim Reprod Sci. 2008;108:334–344
  35. Makaverich AV, Markkula M. Apoptosis and cell proliferation potential of bovine embryos stimulated with insulin-like growth factor I during in vitro maturation and culture. Biol Reprod. 2002;66:386–392
  36. Fisz M. Some probability distributions. In:  De Krieger R editors. Probability Theory and Mathematical Statistics. John Wiley & Sons; 1980;p. 129–174
  37. Sommerfeld V, Niemman H. Cryopreservation of bovine in vitro produced embryos using ethylene glycol in controlled freezing or vitrification. Cryobiology. 1999;38:95–105
  38. Esaki R, Ueda H, Kurome M, Hirakawa K, Tomii R, Yoshioka H, et al. Cryopreservation of porcine embryos derived from in vitro-matured oocytes. Biol Reprod. 2004;71:432–437
  39. Dobrinsky JR. Cellular approach to cryopreservation of embryos. Theriogenology. 1996;45:17–26
  40. Martinez EA, Caamano JN, Gil MA, Rieke A, McCauley T, Cantley TC, et al. Succesful deep uterine embryo transfer in pigs. Theriogenology. 2004;61:137–146
  41. Cuello C, Sanchez-Osorio J, Almiñana C, Gil MA, Perals ML, Lucas X, et al. Effect of the cryoprotectants concentration on the in vitro embryo development and cell proliferation of OPS- vitrified porcine blastocysts. Cryobiology. 2008;56:189–194
  42. Kane MT. Culture media and culture of early embryos. Theriogenology. 1987;27:49–57
  43. Dobrinsky JR, Pursel VG, Long CR, Johnson LA. Birth of piglets after transfer of vitrified of embryos cryopreserved by cytoskeletal stabilization and vitrification. Biol Reprod. 2000;62:564–570
  44. Hall PA, Woods AL. Immunohistochemical markers of cellular proliferation: achievements, problems and prospects. Cell Tissue Kinet. 1993;23:505–522
  45. Fairman M. DNA polymerase delta/PCNA: actions and interactions. J Cell Sci. 1990;95:1–4
  46. Panesku T, Mittal S, Protic M, Oryhon J, Korolev SV, Joachimiak A, et al. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int J Radiat Biol. 2001;77:1007–1021
  47. Vivona JB, Kelman Z. The diverse spectrum of sliding clamp interacting proteins. FEBS Lett. 2003;546:162–172
  48. Markkula M, Räty M, Jauhiainen L, Paranko L, Raula J, Makarevich A. Ratio of proliferating cell nuclear antigen-positive nuclei to total cell number is higher in day 7 than in day 8 vitrified in vitro-produced bovine embryos. Biol Reprod. 2001;65:52–59

PII: S0093-691X(09)00415-4

doi: 10.1016/j.theriogenology.2009.07.031

Theriogenology
Volume 73, Issue 3 , Pages 300-308 , February 2010

Article Tools

* Image Usage & Resolution