Theriogenology
Volume 73, Issue 7 , Pages 839-847 , 15 April 2010

Systematic misestimation of cell subpopulations by flow cytometry: A mathematical analysis

  • A.M. Petrunkina

      Affiliations

    • Cambridge Institute for Medical Research, University of Cambridge, Cambridge, United Kingdom
    • University of Veterinary Medicine Hannover, Unit for Reproductive Medicine of Clinics, Clinic for Horses, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +44 1223762112; fax: +1223 762323.
    • ,
  • R.A.P. Harrison

      Affiliations

    • Great Shelford, Cambridge, United Kingdom

Received 1 May 2009 ,Revised 31 July 2009 ,Accepted 2 September 2009.

References 

  1. Petrunkina AM, Waberski D, Günzel-Apel AR, Töpfer-Petersen E. Determinants of sperm quality and fertility in domestic species. Reproduction. 2007;134:3–17
  2. Eustache F, Jouannet P, Auger J. Evaluation of flow cytometric methods to measure human sperm concentration. J Androl. 2001;22:558–567
  3. Hansen C, Christensen P, Stryhn H, Hedeboe AM, Rode M, Boe-Hansen G. Validation of the FACSCount AF system for determination of sperm concentration in boar semen. Reprod Domest Anim. 2002;37:330–334
  4. Christensen P, Hansen C, Liboriussen T, Lehn-Jensen H. Implementation of flow cytometry for quality control in four Danish bull studs. Anim Reprod Sci. 2005;85:201–208
  5. Prathalingam NS, Holt WW, Revell SG, Jones S, Watson PF. The precision and accuracy of six different methods to determine sperm concentration. J Androl. 2006;27:257–262
  6. Petrunkina AM, Radcke S, Günzel-Apel AR, Harrison RAP, Töpfer-Petersen E. Role of potassium channels, the sodium-potassium pump and the cytoskeleton in the control of dog sperm volume. Theriogenology. 2004;61:35–54
  7. Lu JC, Chen F, Xu HR, Wu YM, Xia XY, Huang YF, et al. Is flow cytometry really adapted to the determination of sperm concentration?. Scand J Clin Lab Invest. 2007;67:394–401
  8. Szász F, Sirivaidyapong S, Cheng FP, Voorhout WF, Marks A, Colenbrander B, et al. Detection of calcium ionophore induced membrane changes in dog sperm as a simple method to predict the cryopreservability of dog semen. Mol Reprod Dev. 2000;55:289–298
  9. Rathi R, Colenbrander B, Bevers MM, Gadella BM. Evaluation of in vitro capacitation of stallion spermatozoa. Biol Reprod. 2001;65:462–470
  10. Petrunkina AM, Volker G, Weitze KF, Beyerbach M, Töpfer-Petersen E, Waberski D. Detection of cooling-induced membrane changes in the response of boar sperm to capacitating conditions. Theriogenology. 2005;63:2278–2299
  11. Petrunkina AM, Volker G, Brandt H, Töpfer-Petersen E, Waberski D. Functional significance of responsiveness to capacitating conditions in boar spermatozoa. Theriogenology. 2005;64:1766–1782
  12. Khalil AAY, Petrunkina AM, Sahin E, Waberski D, Töpfer-Petersen E. Enhanced binding of sperm with superior volume regulation to oviductal epithelium. J Androl. 2006;27:754–765
  13. Harrison RAP, Vickers SE. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil. 1990;88:343–352
  14. Piehler E, Petrunkina AM, Ekhlasi-Hundrieser M, Töpfer-Petersen E. Dynamic quantification of the tyrosine phosphorylation of the sperm surface proteins during capacitation in vitro. Cytometry A. 2006;69:1062–1070
  15. Triphan X, Menzel VA, Petrunkina AM, Cassara MC, Wemheuer W, Hinsch KD, et al. Localization and function of voltage-dependent anion channels (VDAC) in bovine spermatozoa. Eur J Physiol Pflugers Arch. 2008;455:677–686
  16. Nagy S, Jansen J, Topper EK, Gadella BM. A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod. 2003;68:1828–1835
  17. Petrunkina AM, Waberski D, Bollwein H, Sieme H. Identifying non-sperm particles during flow cytometric physiologic assessment: a simple approach. Theriogenology. 2010;73:995–1000

PII: S0093-691X(09)00409-9

doi: 10.1016/j.theriogenology.2009.09.007

Theriogenology
Volume 73, Issue 7 , Pages 839-847 , 15 April 2010

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