Theriogenology
Volume 73, Issue 1 , Pages 97-102 , 1 January 2010

Embryo production after in vitro fertilization with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm

  • S.L. Underwood

      Affiliations

    • Faculty of Veterinary Science, The University of Sydney, Sydney, New South Wales, Australia
    • Corresponding Author InformationCorresponding author. Tel.: +61 2 9351 5832; fax: +61 2 9351 3957.
    • ,
  • R. Bathgate

      Affiliations

    • Faculty of Veterinary Science, The University of Sydney, Sydney, New South Wales, Australia
    • ,
  • D.C. Pereira

      Affiliations

    • Sexing Technologies, Navasota, Texas, USA
    • ,
  • A. Castro

      Affiliations

    • Sexing Technologies, Navasota, Texas, USA
    • ,
  • P.C. Thomson

      Affiliations

    • Faculty of Veterinary Science, The University of Sydney, Sydney, New South Wales, Australia
    • ,
  • W.M.C. Maxwell

      Affiliations

    • Faculty of Veterinary Science, The University of Sydney, Sydney, New South Wales, Australia
    • ,
  • G. Evans

      Affiliations

    • Faculty of Veterinary Science, The University of Sydney, Sydney, New South Wales, Australia

Received 2 April 2009 ,Revised 31 July 2009 ,Accepted 6 August 2009.

References 

  1. Hollinshead FK, Evans G, Evans KM, Catt SL, Maxwell WMC, O’Brien JK. Birth of lambs of a pre-determined sex after in vitro production of embryos using frozen-thawed sex-sorted and re-frozen-thawed ram spermatozoa. Reproduction. 2004;127:557–568
  2. de Graaf SP, Evans G, Maxwell WMC, Cran DG, O’Brien JK. Birth of offspring of pre-determined sex after artificial insemination of frozen-thawed, sex-sorted and re-frozen-thawed ram spermatozoa. Theriogenology. 2007;67:391–398
  3. Underwood SL, Bathgate R, Maxwell WMC, Evans G. In vitro characteristics of frozen-thawed sex-sorted bull spermatozoa after re-freezing or incubation at 15 or 37°C. Theriogenology. 2009;72:1001–1008
  4. Underwood SL, Bathgate R, Maxwell WMC, Evans G. Birth of offspring after artificial insemination of heifers with frozen-thawed, sex-sorted, re-frozen-thawed bull sperm. Anim Reprod Sci 2009; doi:10.1016/j.anireprosci.2009.08.007, in press.
  5. Underwood SL, Bathgate R, Ebsworth M, Maxwell WMC, Evans G. Pregnancy loss in heifers after artificial insemination with frozen-thawed, sex-sorted, re-frozen-thawed dairy bull spermatozoa. Anim Reprod Sci 2009; doi:10.1016/j.anireprosci.2009.06.004, in press.
  6. Puglisi R, Vanni R, Galli A, Balduzzi D, Parati K, Bongiono G, et al. In vitro fertilisation with frozen-thawed bovine sperm sexed by flow cytometry and validated for accuracy by real-time PCR. Reproduction. 2006;132:519–526
  7. Malcolm V, Marfil M, Calvi M, Rigali F, Pugliese M, Gutierrez J, et al. Comparison of in vitro fertilizing capacity of frozen-thawed sex-sorted and sex-sorted frozen-thawed bull spermatozoa. Reprod Fertil Dev. 2007;19:298;[abst.]
  8. Klinc P, Rath D. Reduction of oxidative stress in bovine spermatozoa during flow cytometric sorting. Reprod Domest Anim. 2007;42:63–67
  9. Parrish JJ, Krogenaes A, Susko-Parrish JL. Capacitation of bovine sperm by heparin. Biol Reprod. 1988;38:1171–1180
  10. Holm P, Booth PJ, Schmidt MH, Greve T, Callesen H. High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology. 1999;52:683–700
  11. Xu J, Guo Z, Su L, Nedambale TL, Zhang J, Schenk J, et al. Developmental potential of vitrified Holstein cattle embryos fertilized in vitro with sex-sorted sperm. J Dairy Sci. 2006;89:2510–2518
  12. Cran DG, Cochran DJ, Johnson LA, Wei H, Lu KH, Polge C. Separation of X- and Y-chromosome bearing sperm by flow cytometry for use in IVF. Theriogenology. 1994;41:183;[Abst.]
  13. Merton JS, Haring RM, Stap J, Hoebe RA, Aten JA. Effect of flow cytometrically sorted frozen/thawed semen on success rate of in vitro bovine embryo production [abstract]. Theriogenology. 1997;47:295
  14. Lu KH, Cran DG, Seidel GE. In vitro fertilization with flow-cytometrically-sorted bovine sperm. Theriogenology. 1999;52:1393–1405
  15. Bermejo-Alvarez P, Rizos D, Rath D, Lonergan P, Gutierrez-Adan A. Can bovine in vitro-matured oocytes selectively process X- or Y-sorted sperm differentially?. Biol Reprod. 2008;79:594–597
  16. Zhang M, Lu KH, Seidel GE. Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically sorted, stained and unsorted sperm from different bulls. Theriogenology. 2003;60:1657–1663
  17. Lu KH, Seidel GE. Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow cytometrically-sorted sperm. Theriogenology. 2004;62:819–830
  18. Lonergan P, Fair T. In vitro-produced bovine embryos—dealing with the warts. Theriogenology. 2008;69:17–22

PII: S0093-691X(09)00391-4

doi: 10.1016/j.theriogenology.2009.08.005

Theriogenology
Volume 73, Issue 1 , Pages 97-102 , 1 January 2010

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