Theriogenology
Volume 72, Issue 9 , Pages 1163-1170 , December 2009

Sperm-mediated gene transfer–treated spermatozoa maintain good quality parameters and in vitro fertilization ability in swine

  • M.L. Bacci

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 051 2097912/993; fax: +39 051 2097899.
  • ,
  • A. Zannoni

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
  • ,
  • M. De Cecco

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
  • ,
  • P. Fantinati

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
  • ,
  • C. Bernardini

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
  • ,
  • G. Galeati

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
  • ,
  • M. Spinaci

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
  • ,
  • R.Giovannoni

      Affiliations

    • Department of Surgical Science–DISC, University of Milano Bicocca, Monza, Italy
  • ,
  • M. Lavitrano

      Affiliations

    • Department of Surgical Science–DISC, University of Milano Bicocca, Monza, Italy
  • ,
  • E. Seren

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy
  • ,
  • M. Forni

      Affiliations

    • Department of Veterinary Morphophysiology and Animal Production–DIMORFIPA, University of Bologna, Bologna, Italy

Received 20 March 2009 ,Revised 29 May 2009 ,Accepted 22 June 2009.

References 

  1. Nakanishi A, Iritani A. Gene transfer in the chicken by sperm-mediated methods. Mol Reprod Dev. 1993;36:258–261
  2. Habrova V, Takac M, Navratil J, Macha J, Ceskova N, Jonak J. Association of Rous sarcoma virus DNA with Xenopus laevis spermatozoa and its transfer to ova through fertilization. Mol Reprod Dev. 1996;44:32–42
  3. Arezzo F. Sea-urchin sperm as a vector of foreign genetic information. Cell Biol Int Rep. 1989;13:391–404
  4. Atkinson PW, Hines ER, Beaton S, Matthaei KI, Reed KC, Bradley MP. Association of exogenous DNA with cattle and insect spermatozoa in vitro. Mol Reprod Dev. 1991;29:1–5
  5. Sperandio S, Lulli V, Bacci ML, Forni M, Maione B, Spadafora C, et al. Sperm-mediated DNA transfer in bovine and swine species. Anim Biotechnol. 1996;7:59–77
  6. Lavitrano M, Stoppacciaro A, Bacci ML, Forni M, Fioretti D, Pucci L, et al. HDAF transgenic pigs for xenotransplantation obtained by sperm mediated gene transfer. Transplantation Proc. 1999;31:972–974
  7. Lavitrano M, Bacci ML, Forni M, Lazzereschi D, Di Stefano C, Fioretti D, et al. Efficient production by sperm-mediated gene transfer of human decay accelerating factor (hDAF) transgenic pigs for xenotransplantation. Proc Natl Acad Sci U S A. 2002;99:14230–14235
  8. Webster N, Forni M, Bacci ML, Giovannoni R, Razzini R, Fantinati P, et al. Multi-transgenic pigs expressing three fluorescent proteins produced with high efficiency by sperm mediated gene transfer. Mol Reprod Dev. 2005;72:68–76
  9. Manzini S, Vargiolu A, Stehle IM, Bacci ML, Cerrito MG, Giovannoni R, et al. Genetically modified pigs produced with a non viral episomal vector. Proc Natl Acad Sci U S A. 2006;103:17672–17677
  10. Fantinati P, Zannoni A, Bernardini C, Webster N, Lavitrano M, Forni M, et al. Laparoscopic insemination technique with low numbers of spermatozoa in superovulated prepuberal gilts for biotechnological application. Theriogenology. 2005;63:806–817
  11. Tummaruk P, Lundeheim N, Einarsson S, Dalin AM. Effect of birth litter size, birth parity number, growth rate, backfat thickness and age at first mating of gilts on their reproductive performance as sows. Anim Reprod Sci. 2001;66:225–237
  12. Fantinati P, Zannoni A, Bernardini C, Forni M, Tattini A, Seren A, et al. Evaluation of swine fertilisation medium (SFM) efficiency in preserving spermatozoa quality during long-term storage in comparison to four commercial swine extenders. Animal. 2008;3:269–274
  13. Maione B, Pittoggi C, Achene L, Lorenzini R, Spadafora C. Activation of endogenous nucleases in mature sperm cells upon interaction with exogenous DNA. DNA Cell Biol. 1997;16:1087–1097
  14. Lavitrano M, Forni M, Bacci ML, Di Stefano C, Varzi V, Wang H, et al. Sperm mediated gene transfer in pig: selection of donor boars and optimization of DNA uptake. Mol Reprod Dev. 2003;64:284–291
  15. Spinaci M, De Ambrogi M, Volpe S, Galeati G, Tamanini C, Seren E. Effect of staining and sorting on boar sperm membrane integrity, mitochondrial activity and in vitro blastocyst development. Theriogenology. 2005;64:191–201
  16. Xia XY, Wu YM, Hou BS, Yang B, Pan LJ, Shi YC, et al. Evaluation of sperm mitochondrial membrane potential by JC-1 fluorescent staining and flow cytometry. Zhonghua Nan Ke Xue. 2008;14:135–138
  17. Huo LJ, Ma XH, Yang ZM. Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage. Theriogenology. 2002;58:1349–1360
  18. Spinaci M, Volpe S, De Ambrogi M, Tamanini C, Galeati G. Effects of epigallocatechin-3-gallate (EGCG) on in vitro maturation and fertilization of porcine oocytes. Theriogenology. 2008;69:877–885
  19. Funahashi H, Cantley T, Day BN. Synchronization of meiosis in porcine oocytes by exposure to dibutyryl cyclic adenosine monophosphate improves developmental competence following in vitro fertilization. Biol Reprod. 1997;57:49–53
  20. Petters RM, Wells KD. Culture of pig embryos. J Reprod Fertil Suppl. 1993;48:61–73
  21. Carballada R, Degefa T, Esponda P. Transfection of mouse eggs and embryos using DNA combined to cationic liposomes. Mol Reprod Dev. 2000;56:360–365
  22. Johnson LA, Weitze KF, Fiser P. Maxwell WMC. Storage of boar semen. Anim Reprod Sci. 2000;62:143–172
  23. Kang JH, Hakimov H, Ruiz A, Friendship RM, Buhr M, Golovan SP. The negative effects of exogenous DNA binding on porcine spermatozoa are caused by removal of seminal fluid. Theriogenology. 2008;70:1288–1296
  24. Anzar M, Buhr MM. Spontaneous uptake of exogenous DNA by bull spermatozoa. Theriogenology. 2006;65:683–690
  25. Hoelker M, Mekchay S, Schneider H, Bracket BG, Tesfaye D, Jennen D, et al. Quantification of DNA binding, uptake, transmission and expression in bovine sperm mediated gene transfer by RT-PCR: effect of transfection reagent and DNA architecture. Theriogenology. 2007;67:1097–1107
  26. Bacci ML. A brief overview of transgenic farm animals. Vet Res Commun. 2007;31(Suppl 1):9–14

PII: S0093-691X(09)00344-6

doi: 10.1016/j.theriogenology.2009.06.033

Theriogenology
Volume 72, Issue 9 , Pages 1163-1170 , December 2009