Theriogenology
Volume 69, Issue 9 , Pages 1129-1138 , June 2008

Assessment of motility of ejaculated, liquid-stored boar spermatozoa using computerized instruments

  • F. Tejerina

      Affiliations

    • Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Ullsväg 14C, Box 7054, SE-750 07 Uppsala, Sweden
    • Department of Medicine, Surgery, and Veterinary Anatomy, Section of Medicine and Surgery, Faculty of Veterinary Medicine, University of Leon, CP 24071 Leon, Spain
  • ,
  • K. Buranaamnuay

      Affiliations

    • Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Ullsväg 14C, Box 7054, SE-750 07 Uppsala, Sweden
    • Department of Obstetrics, Gynecology, and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, 10330 Bangkok, Thailand
  • ,
  • F. Saravia

      Affiliations

    • Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Ullsväg 14C, Box 7054, SE-750 07 Uppsala, Sweden
  • ,
  • M. Wallgren

      Affiliations

    • Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Ullsväg 14C, Box 7054, SE-750 07 Uppsala, Sweden
  • ,
  • H. Rodriguez-Martinez

      Affiliations

    • Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Ullsväg 14C, Box 7054, SE-750 07 Uppsala, Sweden
    • Corresponding Author InformationCorresponding author at: Division of Reproduction, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Ullsväg 14C, Box 7054, SE-750 07 Uppsala, Sweden. Tel.: +46 1867 2172; fax: +46 1867 3545.

Received 14 September 2007 ,Revised 19 November 2007 ,Accepted 12 January 2008.

References 

  1. Rodriguez-Martinez H. State of the art in farm animal sperm evaluation. Reprod Fertil Dev. 2007;19:91–101
  2. Januskauskas A, Johannisson A, Rodríguez-Martínez H. Assessment of sperm quality through fluorometry and sperm chromatin structure assay in relation to field fertility of frozen–thawed semen from Swedish AI bulls. Theriogenology. 2001;55:947–961
  3. Peña FJ, Saravia F, Nuñez-Martínez I, Johannisson A, Walgren M, Rodríguez-Martínez H. Do different portions of the boar ejaculate vary in their ability to sustain cryopreservation?. Anim Reprod Sci. 2006;93:101–113
  4. Gadea J. El uso de semen porcino congelado (use of frozen porcine semen). Mundo Ganadero. 2004;169:60–62[in Spanish]
  5. Amann RP. Can the fertility potential of a seminal sample be predicted accurately?. J Androl. 1989;10:89–98
  6. Brazil C, Swan SH, Tollner CR, Treece C, Drobnis EZ, Wang C, et al. Quality control of laboratory methods for semen evaluation in a multicenter research study. J Androl. 2004;25:645–656
  7. Jorgensen N, Auger J, Giwercman A, Irvine DS, Jensen TK, Jouannet P, et al. Semen analysis performed by different laboratory teams: an intervariation study. Int J Androl. 1997;20:201–208
  8. Yeung CH, Cooper TG, Nieschlag E. A technique for standardization and quality control of subjective sperm motility assessments in semen analysis. Fertil Steril. 1997;67:1156–1158
  9. Verstegen J, Iguer-Ouada M, Onclin K. Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology. 2002;57:149–179
  10. Katkov II, Lulat AG. Do conventional CASA-parameters reflect recovery of kinematics after freezing? “CASA paradox” in the analysis of recovery of spermatozoa after cryopreservation. Cryo Lett. 2000;21:141–148
  11. Abaigar T, Holt WV, Harrison RA, del Barrio G. Sperm subpopulations in boar (Sus scrofa) and gazelle (Gazella dama mhorr) as revealed by pattern analysis of computer-assisted motility assessments. Biol Reprod. 1999;60:32–41
  12. Quintero-Moreno A, Rigau T, Rodriguez-Gil JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology. 2004;61:673–690
  13. Cremades T, Roca J, Rodriguez-Martinez H, Abaigar T, Vazquez JM, Martinez EA. Kinematic changes during the cryopreservation of boar spermatozoa. J Androl. 2005;26:610–618
  14. Saravia F, Hernández M, Wallgren MK, Johannisson A, Rodríguez-Martínez H. Cooling during semen cryopreservation does not induce capacitation of boar spermatozoa. Int J Androl. 2007;30:485–499
  15. Mortimer ST, Maxwell WM. Kinematic definition of ram sperm hyperactivation. Reprod Fertil Dev. 1999;11:25–30
  16. Schmidt H, Kamp G. Induced hyperactivity in boar spermatozoa and its evaluation by computer-assisted sperm analysis. Reproduction. 2004;128:171–179
  17. Holt W, Watson P, Curry M, Holt C. Reproducibility of computer-aided semen analysis: comparison of five different systems used in a practical workshop. Fertil Steril. 1994;62:1277–1282
  18. Rodríguez-Martínez H, Larson B, Zhang BR, Söderquist L. In vitro assessment of viability and fertilizing capacity of bull spermatozoa. J Reprod Dev. 1997;43:1–11
  19. Tardif S, Laforest JP, Cormier N, Bailey JL. The importance of porcine sperm parameters on fertility in vivo. Theriogenology. 1999;52:447–459
  20. Iguer-Ouada M, Verstegen JP. Evaluation of the “Hamilton Thorn computer-based automated system” for dog semen analysis. Theriogenology. 2001;55:733–749
  21. Eriksson BM, Rodriguez-Martinez H. Effect of freezing and thawing rates on the post-thaw viability of boar spermatozoa frozen in FlatPacks and Maxi-straws. Anim Reprod Sci. 2000;63:205–220
  22. Saravia F, Wallgren M, Nagy S, Johannisson A, Rodriguez-Martinez H. Deep freezing of concentrated boar semen for intra-uterine insemination: effects on sperm viability. Theriogenology. 2005;63:1320–1333
  23. Simonsson A. Näringsrekommendationer och fodermedel till svin (nutritional recommendations and feedstuff for swine). vol. 75. Uppsala Husdjur: Swedish University of Agricultural Sciences. Research Information Center; 1994;p. 71–84 [in Swedish]
  24. Hancock JL, Hovell GJR. The collection of boar semen. Vet Rec. 1959;71:664–665
  25. Owen DH, Katz DF. Sampling factor influencing accuracy of sperm kinematic analysis. J Androl. 1993;14:210–221
  26. White IG. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev. 1993;5:639–658
  27. Watson PF. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev. 1995;7:871–891
  28. De Ambrogi M, Ballester J, Saravia F, Caballero I, Johannisson A, Wallgren M, et al. Effect of storage in short- and long-term commercial semen extenders on the motility, plasma membrane, and chromatin integrity of boar spermatozoa. Int J Androl. 2006;29:543–552
  29. Dubé C, Beaulieu M, Reyes-Moreno C, Guillemette C, Bailey J. Boar sperm storage capacity of BTS and Androhep Plus: viability, motility, capacitation, and tyrosine phosphorylation. Theriogenology. 2004;62:874–886
  30. Huo LJ, Ma XH, Yang ZM. Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage. Theriogenology. 2002;58:1349–1360
  31. Mortimer D, Goel N, Shu MA. Evaluation of the CellSoft automated semen analysis system in a routine laboratory setting. Fertil Steril. 1988;50:960–968
  32. Wetzels AM, Janssen HJ, Goverde HJ, Bastiaans BA, Takahashi K, Rolland R. The influence of sperm density on the motility characteristics of washed human spermatozoa. Int J Androl. 1993;16:15–19
  33. Peña FJ, Johannisson A, Wallgren M, Rodríguez-Martínez H. Assessment of fresh and frozen–thawed boar semen using an Annexin-V assay: a new method of evaluating sperm membrane integrity. Theriogenology. 2003;60:677–689

PII: S0093-691X(08)00073-3

doi: 10.1016/j.theriogenology.2008.01.027

Theriogenology
Volume 69, Issue 9 , Pages 1129-1138 , June 2008