Theriogenology
Volume 69, Issue 9 , Pages 1083-1094 , June 2008

Freeze-thawing induces alterations in the protamine-1/DNA overall structure in boar sperm

  • E. Flores

      Affiliations

    • Unit of Animal Reproduction, Department Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Spain
    • ,
  • D. Cifuentes

      Affiliations

    • Department Biochemistry and Molecular Biology and IRRB, Barcelona Science Park, University of Barcelona, Barcelona, Spain
    • ,
  • J.M. Fernández-Novell

      Affiliations

    • Department Biochemistry and Molecular Biology and IRRB, Barcelona Science Park, University of Barcelona, Barcelona, Spain
    • ,
  • A. Medrano

      Affiliations

    • Unit of Animal Reproduction, Department Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Spain
    • ,
  • S. Bonet

      Affiliations

    • Unit of Biotechnology of Porcine Reproductión, University of Girona, Girona, Spain
    • ,
  • M.D. Briz

      Affiliations

    • Unit of Biotechnology of Porcine Reproductión, University of Girona, Girona, Spain
    • ,
  • E. Pinart

      Affiliations

    • Unit of Biotechnology of Porcine Reproductión, University of Girona, Girona, Spain
    • ,
  • A. Peña

      Affiliations

    • Unit of Animal Reproduction, Department Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Spain
    • ,
  • T. Rigau

      Affiliations

    • Unit of Animal Reproduction, Department Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Spain
    • ,
  • J.E. Rodríguez-Gil

      Affiliations

    • Unit of Animal Reproduction, Department Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, E-08193 Bellaterra, Spain
    • Corresponding Author InformationCorresponding author. Tel.: +34 935811045; fax: +34 935812006.

Received 1 October 2007 ,Revised 28 November 2007 ,Accepted 23 January 2008.

References 

  1. Holt WV, North RD. Effects of temperature and restoration of osmotic equilibrium during thawing on the induction of plasma-membrane damage in cryopreserved ram spermatozoa. Biol Reprod. 1994;51:414–424
  2. Mohammad SN, Barratt CL, Cooke ID, Moore HD. Continuous assessment of human spermatozoa viability during cryopreservation. J Androl. 1997;18:43–50
  3. Medrano A, Watson PF, Holt WV. Importance of cooling rate and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Reproduction. 2002;123:315–322
  4. Shekarriz M, De Wire DM, Thomas AJ, Agarwal A. A method of human semen centrifugation to minimize the iatrogenic sperm injuries caused by reactive oxygen species. Eur Urol. 1995;28:31–35
  5. Carvajal G, Cuello C, Ruiz M, Vázquez JM, Martínez EA, Roca J. Effects of centrifugation before freezing on boar sperm cryosurvival. J Androl. 2004;25:389–396
  6. Brouwers JF, Silva PFN, Gadella BM. New assays for detection and localization of endogenous lipid peroxidation products in living boar sperm after BTS dilution or after freezing–thawing. Theriogenology. 2005;63:458–469
  7. Silva PFN, Gadella BM. Detection of damage in mammalian sperm cells. Theriogenology. 2006;65:958–978
  8. Baumber J, Ball BA, Linfor JJ, Meyers SA. Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa. J Androl. 2003;24:621–628
  9. Fraser L, Strezeżek J. Effects of freezing–thawing on DNA integrity of boar spermatozoa assessed by the Neutral Comet Assay. Reprod Domest Anim. 2005;40:530–536
  10. Evenson DP, Thompson L, Jost L. Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility. Theriogenology. 1994;41:637–651
  11. Hernández M, Roca J, Ballester J, Vázquez JM, Martínez EA, Johannisson A, et al. Differences in SCSA outcome among boars with different sperm freezability. J Androl. 2006;29:583–591
  12. Martínez-Pastor F, Johanisson A, Gil J, Kaabi M, Anel L, Paz P, et al. Use of chromatin stability assay, mitochondrial stain JC-1 and fluorometric assessment of plasma membrane to evaluate frozen–thawed ram semen. An Reprod Sci. 2004;84:121–133
  13. Wykes SM, Krawetz A. The structural organization of sperm chromatin. J Biol Chem. 2003;278:29471–29474
  14. O’Brien J, Zini A. Sperm DNA integrity and male infertility. Urology. 2005;65:16–22
  15. Biegeleisen K. The probable structure of the protamine–DNA complex. J Theor Biol. 2006;241:533–540
  16. Aoki VW, Liu L, Carrell DT. Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males. Hum Reprod. 2005;20:1298–1306
  17. Aoki VW, Moskovtsev SI, Willis J, Liu L, Mullen JBM, Carrell DT. DNA integrity is compromised in protamine-deficient human sperm. J Androl. 2005;26(3):741–748
  18. Shimada A, Kikuchi K, Noguchi J, Akina K, Nakano M, Kaneko H. Protamine dissociation before decondensation of sperm nuclei during “in vitro” fertilization of pig oocytes. J Reprod Fertil. 2000;120:247–256
  19. Nakazawa Y, Shimada A, Noguvhi J, Domeki I, Kaneko H, Kikuchi K. Replacement of nuclear protein by histone in pig sperm during “in vitro” fertilization. Reproduction. 2002;124:565–572
  20. Eriksson BM, Rodriguez-Martinez H. Effect of freezing and thawing rates on the post-thaw viability of boar spermatozoa frozen in FlatPacks and Maxi-straws. Anim Reprod Sci. 2000;63:205–220
  21. Graham EF, Rajamannan AHJ, Schmehl MKL, Maki-Laurila M, Bower RE. Fertility studies with frozen boar spermatozoa. AI Digest. 1971;19:61
  22. Saravia F, Wallgren M, Nagy S, Johannisson A, Rodríguez-Martínez H. Deep freezing of concentrated boar semen for intra-uterine insemination: effects on sperm viability. Theriogenology. 2005;63:1320–1333
  23. Bussalleu E, Pinart E, Yeste M, Briz M, Sancho S, Garcia-Gil N, et al. Development of a protocol for multiple staining with fluorochromes to assess the functional status of boar spermatozoa. Microsc Res Tech. 2005;68:277–283
  24. Rodríguez-Gil JE, Rigau T. Effects of slight agitation on the quality of refrigerated boar sperm. Anim Reprod Sci. 1995;39:141–146
  25. Quintero-Moreno A, Rigau T, Rodríguez-Gil JE. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology. 2004;61:673–690
  26. Fraser L, Parda A, Strzežec J. Comparisons of the neutral comet assay (NCA) and Sperm–Sus–Halomax® kit in relation to DNA integrity of frozen–thawed boar spermatozoa. Reprod Domest Anim. 2007;42(Suppl. 2):65
  27. Enciso M, López-Fernández C, Fernández JL, García P, Gosálbez Ai Gosálvez J. A new method to analyze boar sperm DNA fragmentation under bright-field or fluorescence microscopy. Theriogenology. 2006;65:308–316
  28. Powell R, Gannon F. Purification of DNA by phenol extraction and ethanol precipitation. In: Oxford practical approach series. Oxford University Press, 2002. Oxford protocol online. http://fds.oup.com/www.oup.co.uk/pdf/pas/9v1-7-3.pdf.
  29. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;15:680–685
  30. Burnett WN. “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981;112:195–203
  31. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem. 1976;72:248–254
  32. SAS. SAS/SATC Software. Cary, NC, USA: SAS Institute Inc.; 1996.
  33. De Ambrogi M, Spinaci M, Galeati G, Tamanini C. Viability and DNA fragmentation in differently sorted boar spermatozoa. Theriogenology. 2006;66:1994–2000
  34. Smith R, Kaune H, Parodi P, Madariaga M, Rios R, Morales I, et al. Increased sperm DNA damage in patients with varicocele: relationship with seminal oxidative stress. Hum Reprod. 2005;21:986–993
  35. Boe-Hansen GB, Ersbøll AK, Greve T, Christensen P. Increasing storage time of extended boar semen reduces sperm DNA integrity. Theriogenology. 2005;63:2006–2019
  36. De Ambrogi M, Ballester J, Saravia F, Caballero I, Johanisson A, Wallgren M, et al. Effect of storage in short- and long-term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa. Int J Androl. 2006;29:543–552
  37. Flesch FM, Brouwers JF, Nievelstein PF, Verkleij AJ, van Golde LM, Colenbrander B, et al. Bicarbonate stimulated phospholipid scrambling induces cholesterol redistribution and enables cholesterol depletion in the sperm plasma membrane. J Cell Sci. 2001;114:3543–3555
  38. Breininger E, Beorlegui NB, O’Flaherty CM, Beconi MT. Alpha-tocopherol improves biochemical and dynamic parameters in cryopreserved boar semen. Theriogenology. 2005;63:2126–2135
  39. Holt WV, Medrano A, Thurston LM, Watson PF. The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology. 2005;63:370–382

PII: S0093-691X(08)00068-X

doi: 10.1016/j.theriogenology.2008.01.022

Theriogenology
Volume 69, Issue 9 , Pages 1083-1094 , June 2008

Article Tools

* Image Usage & Resolution