Theriogenology
Volume 61, Issue 6 , Pages 1193-1201 , 15 April 2004

Effects of varying the holding temperature and interval from collection to freezing on post-thaw development of bovine embryos in vitro

  • F.D Jousan

      Affiliations

    • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0306, USA
    • ,
  • M.D Utt

      Affiliations

    • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0306, USA
    • ,
  • S.S Whitman

      Affiliations

    • Ashby Embryos, Harrisonburg, VA 22801, USA
    • ,
  • R.H Hinshaw

      Affiliations

    • Ashby Embryos, Harrisonburg, VA 22801, USA
    • ,
  • W.E Beal

      Affiliations

    • Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0306, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-540-231-4750; fax: +1-540-231-3010.

Received 14 January 2003 ,Accepted 14 July 2003.

References 

  1. Leibo SP. Cryobiology: preservation of mammalian embryos. Basic Life Sci. 1986;37:251–272
  2. Pettit WH. Commercial freezing of bovine embryos in glass ampules. Theriogenology. 1985;23:13–16
  3. Wright JM. Commercial freezing of bovine embryos in straws. Theriogenology. 1985;23:17–29
  4. Hasler JF. Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle. Theriogenology. 2001;58:1401–1415
  5. Dochi O, Yamamoto Y, Saga H, Yoshiba N, Kano N, Maeda J, et al.  Direct transfer of bovine embryos frozen–thawed in the presence of propylene glycol or ethylene glycol under on-farm conditions in an integrated ET program. Theriogenology. 1998;49:1051–1058
  6. Martinez AG, Brogliatti GM, Valcarcel A, de las Heras MA. Pregnancy rates after transfer of frozen bovine embryos: a field trial. Theriogenology. 2003;58:963–972
  7. Jurgens MH. Animal feeding and nutrition. Dubuque, IA: Kendall/Hunt Publishing Co.; 1997.
  8. International Embryo Transfer Society. Manual of the international ET society. 3rd ed. Savoy, IL, USA; 1998.
  9. SAS. Statistical analysis system: a user’s guide. Version 8.02, 4th ed. Cary, NC, USA: Statistical Analysis System Institute, Inc.; 2001.
  10. Hasler JF. Current status and potential of embryo transfer and reproductive technology in dairy cattle. J. Dairy Sci. 1992;75:2857–2879

PII: S0093-691X(03)00304-2

doi: 10.1016/j.theriogenology.2003.07.009

Theriogenology
Volume 61, Issue 6 , Pages 1193-1201 , 15 April 2004

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